文献类型：期刊文献

英文题名：The Osmotin-Like Protein Gene PdOLP1 Is Involved in Secondary Cell Wall Biosynthesis during Wood Formation in Poplar

作者：Li, Shaofeng[1] Zhang, Yaoxiang[1] Xin, Xuebing[1] Ding, Changjun[2] Lv, Fuling[1] Mo, Wenjuan[1] Xia, Yongxiu[1] Wang, Shaoli[1] Cai, Jingyan[1] Sun, Lifang[1] Du, Manyi[1] Dong, Chenxi[1] Gao, Xu[1] Dai, Xinlu[1] Zhang, Jianhui[3] Sun, Jinshuang[1]

通信作者：Sun, JS[1];Zhang, JH[2]

机构：[1]Chinese Acad Forestry, Expt Ctr Forestry North China, Beijing 100023, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat,State Forestry A, Beijing 100091, Peoples R China;[3]North Carolina Cent Univ, Biomfg Res Inst & Technol Enterprise, Dept Pharmaceut Sci, Durham, NC 27707 USA

年份：2020

卷号：21

期号：11

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000543400300255)】；

基金：This work was supported by the National Key Program on Transgenic Research (Grant No. 2018ZX08020002), the National Natural Science Foundation of China (Grant No. 31700531, 31770705 and 31400570), and the Open Fund of State Key Laboratory of Tree Genetics and Breeding (Chinese Academy of Forestry) (Grant No. TGB2013002).

语种：英文

外文关键词：functional mechanisms; negative regulator; PdOLP1; Populus deltoides; secondary wall biosynthesis

摘要：Osmotin-like proteins (OLPs) mediate defenses against abiotic and biotic stresses and fungal pathogens in plants. However, no OLPs have been functionally elucidated in poplar. Here, we report an osmotin-like protein designated PdOLP1 from Populus deltoides (Marsh.). Expression analysis showed that PdOLP1 transcripts were mainly present in immature xylem and immature phloem during vascular tissue development in P. deltoides. We conducted phenotypic, anatomical, and molecular analyses of PdOLP1-overexpressing lines and the PdOLP1-downregulated hybrid poplar 84K (Populus alba x Populus glandulosa) (Hybrid poplar 84K PagOLP1, PagOLP2, PagOLP3 and PagOLP4 are highly homologous to PdOLP1, and are downregulated in PdOLP1-downregulated hybrid poplar 84K). The overexpression of PdOLP1 led to a reduction in the radial width and cell layer number in the xylem and phloem zones, in expression of genes involved in lignin biosynthesis, and in the fibers and vessels of xylem cell walls in the overexpressing lines. Additionally, the xylem vessels and fibers of PdOLP1-downregulated poplar exhibited increased secondary cell wall thickness. Elevated expression of secondary wall biosynthetic genes was accompanied by increases in lignin content, dry weight biomass, and carbon storage in PdOLP1-downregulated lines. A PdOLP1 coexpression network was constructed and showed that PdOLP1 was coexpressed with a large number of genes involved in secondary cell wall biosynthesis and wood development in poplar. Moreover, based on transcriptional activation assays, PtobZIP5 and PtobHLH7 activated the PdOLP1 promoter, whereas PtoBLH8 and PtoWRKY40 repressed it. A yeast one-hybrid (Y1H) assay confirmed interaction of PtoBLH8, PtoMYB3, and PtoWRKY40 with the PdOLP1 promoter in vivo. Together, our results suggest that PdOLP1 is a negative regulator of secondary wall biosynthesis and may be valuable for manipulating secondary cell wall deposition to improve carbon fixation efficiency in tree species.