文献类型：期刊文献

英文题名：Identification of the RNA m5C methyltransferase genes in Populus alba x Populus glandulosa and the role of PagTRM4B in wood formation

作者：Chen, Jiahuan[1] Qi, Guoning[2,3] Zhong, Minyi[2] Geng, Zhenghao[1] Chen, Jingran[2] Zhao, Jie[4] Chen, Mingjia[4] Yan, Xiaojing[1]

通信作者：Yan, XJ[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Zhejiang A&F Univ, State Key Lab Dev & Utilizat Forest Food Resources, Hangzhou 311300, Peoples R China;[3]Zhejiang A&F Univ, Prov Key Lab Nonwood Forest & Qual Control & Utili, Hangzhou 311300, Peoples R China;[4]Nanjing Agr Univ, Coll Life Sci, Nanjing 210095, Peoples R China

年份：2025

卷号：5

外文期刊名：FORESTRY RESEARCH

收录：WOS:【ESCI(收录号:WOS:001614059900001)】；

基金：Acknowledgments This work was supported by the National Key Research and Deve-lopment Program of China (2021YFD2200900 to Xiaojing Yan) , and Fundamental Research Funds of Chinese Academy of Forestry (CAFYBB2023QB010 to Xiaojing Yan) .

语种：英文

摘要：N-5-methylcytosine (m(5)C) RNA methylation plays an essential role in gene regulation, yet its functions in woody plant development remain poorly understood. This study systematically identified 12 putative tRNA-specific methyltransferase 4 (TRM4) homologs (PagTRM4s) within the P. alba x P. glandulosa genome. Phylogenetic and synteny analyses revealed high evolutionary conservation across Arabidopsis and rice. Structural and domain analyses suggested functional conservation among allelic pairs. Expression profiling showed that PagTRM4B-a maintained stable expression in xylem at different developmental stages. PagTRM4B-a overexpression in poplar resulted in decreased plant height and stem diameter, modified wood composition, and increased RNA m(5)C levels. Histological analysis showed that overexpression enhanced xylem cell expansion and secondary xylem formation without affecting cambial activity. Moreover, the expression levels of secondary cell wall (SCW) biosynthesis genes were significantly down-regulated in PagTRM4B-a-OE transgenic plants. This study offers new insights into the epigenetic control of secondary growth in trees and highlights PagTRM4B-a as a potential target for influencing wood formation in woody plants.