文献类型：期刊文献

英文题名：A MADS-box gene of Populus deltoides expressed during flower development and in vegetative organs

作者：Zhang, Bingyu[1] Su, Xiaohua[1] Zhou, Xiangming[2]

第一作者：张冰玉

通信作者：Zhang, BY[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Forest Adm, Lab Tree Breeding & Cultivat, Beijing 100091, Peoples R China;[2]Tianjin Res Ctr Agr Biotechnol, Tianjin 300192, Peoples R China

年份：2008

卷号：28

期号：6

起止页码：929-934

外文期刊名：TREE PHYSIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000256711500011)】；

语种：英文

外文关键词：floral development; relative-quantitative real-time PCR; transcription factor; transgene flow

摘要：A MADS-box gene (PdPI) was isolated from a cDNA library constructed from male flower buds of Populus deltoides Bartr. ex Marsh. According to an analysis of genomic DNA structure and putative protein structure, and a phylogenetic study, PdPI is an ortholog of the Arabidopsis PI gene. Relative-quantitative real-time polymerase chain reaction analysis showed that PdPI has a broader expression pattern than PI in Arabidopsis. PdPI was strongly expressed in floral buds and roots and weakly expressed in immature xylem, leaves and apical buds of the male P. deltoides tree. In male inflorescences, PdPI expression was abundant in the perianth and anther, and weak in the peduncle and mature pollen. The large differences in PdPI expression at various phases of male floral bud development were closely related to the development of flower organs (perianth and stamen) and pollen. PdPI was also expressed in female inflorescences. Our results suggest that PdPI has multiple functions in the development of P. deltoides.