文献类型：期刊文献

英文题名：Applicability of chloroplast DNA barcodes for wood identification between Santalum album and its adulterants

作者：Jiao, Lichao[1,2] He, Tuo[1,2] Dormontt, Eleanor E.[3] Zhang, Yonggang[1,2] Lowe, Andrew J.[3] Yin, Yafang[1,2]

第一作者：焦立超

通信作者：Yin, YF[1];Yin, YF[2]

机构：[1]Chinese Acad Forestry, Chinese Res Inst Wood Ind, Dept Wood Anat & Utilizat, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Wood Collect WOODPEDIA, Beijing 100091, Peoples R China;[3]Univ Adelaide, Sch Biol Sci, Identificat & Forens Facil, Adelaide, SA 5005, Australia

年份：2019

卷号：73

期号：2

起止页码：209-218

外文期刊名：HOLZFORSCHUNG

收录：;EI(收录号：20190606485663);Scopus(收录号：2-s2.0-85061162528);WOS:【SCI-EXPANDED(收录号:WOS:000457473400010)】；

基金：This work was supported financially by National Natural Science Foundation of China (Funder Id: 10.13039/501100001809; Grant No. 31600451), Fundamental Research Funds of CAF (Grant No. CAFYB-B2017ZE003) and National High-level Talents Special Support Plan (Ten-Thousand Talents Program) of China (No. W02020331).

语种：英文

外文关键词：barcode combinations psbA-trnH and trnK; DNA barcode; illegal logging; neighbor-joining (NJ) method; sandal wood; species identification; TaxonDNA method

摘要：The East Indian sandalwood, Santalum album L., is known for its fragrant heartwood and extractive oils. The increasing demand for the valuable sandalwood products has led to illegal and excessive logging, and there are other Santalum species in the commercial market as substitutes (adulterants). Improved tools for the identification of Santalum species are needed to control this situation. In this study, four chloroplast DNA (cpDNA) regions were screened (matK , psbA-trnH, trnK and trnL) on 35 vouchered specimens covering five Santalum species, i.e. Santalum acuminatum, S. album, Santalum lanceolatum, Santalum murrayanum and Santalum spicatum. The goal of this study was to test the species discrimination ability by means of both the TaxonDNA and neighbor-joining (NJ) methods. The results indicate that the combination of psbA-trnH+ trnK gave the best performance for discrimination (100%) of the studied Santalum species concerning the discrimination ability and recovery rate. Meanwhile, six unvouchered wood specimens were retrieved and accurately identified at the species level based on the recommended DNA barcodes. The DNA barcode method is now ready for application in the monitoring of the trade of this valuable resource, and provides an effective approach for wood species identification and product authentication.