详细信息
云南省核桃品种遗传多样性的FISH-AFLP分析 被引量:16
FISH-AFLP Analysis of Genetic Diversity on Walnut Cultivars in Yunnan Province
文献类型:期刊文献
中文题名:云南省核桃品种遗传多样性的FISH-AFLP分析
英文题名:FISH-AFLP Analysis of Genetic Diversity on Walnut Cultivars in Yunnan Province
作者:宁德鲁[1] 马庆国[2] 张雨[1] 王滑[2] 陆斌[3] 裴东[2]
第一作者:宁德鲁
机构:[1]云南省林业科学院经济林研究所,云南昆明650204;[2]中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京100091;[3]云南省林业技术推广总站,云南昆明650224
年份:2011
卷号:24
期号:2
起止页码:189-193
中文期刊名:林业科学研究
外文期刊名:Forest Research
收录:CSTPCD;;Scopus;北大核心:【北大核心2008】;CSCD:【CSCD2011_2012】;
基金:林业公益性行业科研专项经费项目:核桃优异种质资源保存与开发利用技术研究(200704046),核桃集约及可持续生产关键技术研究(201004048)
语种:中文
中文关键词:核桃;品种;遗传多样性;FISH-AFLP;聚类分析
外文关键词:walnut(Juglans sigillata Dode); cultivar; genetic diversity; FISH-AFLP; cluster analysis
分类号:S792.13
摘要:采用FISH-AFLP技术,选取8对E+3和M+3引物组合,对云南省林木品种审定委员会审(认)定的17个核桃品种(无性系)进行了基因组DNA分子水平上的检测,实验结果共获得987条可统计的条带,其中942条呈多态性,多态性带百分率达95.44%;17份供试核桃样品的遗传相似系数范围为0.606 9 0.796 4,平均为0.734 9。本研究分析了云南省审(认)定核桃品种(无性系)的遗传多样性和亲缘关系,建立了它们的指纹图谱,为下一步的核桃新品种选育、构建核桃优良种质库提供了理论指导。
The genetic diversities of 17 walnut cultivars(Juglans sigillata Dode) authorized or approved by Yunnan Province Forest Tree Cultivar Registration Committee were investigated with FISH-AFLP technical system.Eight pairs of E+3/M+3 primers were used to amplify the genomic DNA.The results showed that 987 AFLP bands were obtained,942 of them were polymorphic markers and the average percent of polymorphic bands was 95.44%.The genetic similarity indexes among all the 17 cultivars are ranged from 0.606 9 to 0.796 4 with an average of 0.734 9.These investigations would be significant in utilizing walnut germplasm resources and cultivating new varieties.
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