文献类型：期刊文献

英文题名：Callus-mediated and direct protocorm-like body formation of Bletilla striata and assessment of clonal fidelity using ISSR markers

作者：Wang, C. X.[1] Tian, M.[1]

第一作者：王彩霞

通信作者：Wang, CX[1]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Fuyang 311400, Zhejiang, Peoples R China

年份：2014

卷号：36

期号：9

起止页码：2321-2330

外文期刊名：ACTA PHYSIOLOGIAE PLANTARUM

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000340584400004)】；

基金：Financial support by the Fundamental Research Funds for the Central Non-profit Research Institutes of RISF-CAF (RISF6157) is gratefully acknowledged.

语种：英文

外文关键词：Bletilla striata; Callus; Micropropagation; Protocorm-like body (PLB); ISSR marker

摘要：Two efficient morphogenetic pathways for micropropagation of Bletilla striata (Thunb.) Reichb. f. have been established through the callus-mediated and direct formation of protocorm-like bodies (PLBs) from protocorms and shoot tips. Green calli were induced from the basal surface of protocorms and the cut-end of shoot tips on Vacin and Went (VW) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) or alpha-naphthalene acetic acid (NAA) after 3-5 weeks, with the highest frequency of explants forming callus (48.0 %) from protocorms at 1.0 mg l(-1) 2,4-D. The calli obtained from all plant growth regulator (PGR) treatments could proliferate and differentiate PLBs on the PGR-free medium. NAA and 2,4-D significantly enhanced the growth of callus. The fastest growth rate of callus was achieved at the combination of 1.0 mg l(-1) 2,4-D and 1.0 mg l(-1) TDZ with 46.2-fold within 3 months. The regeneration of PLBs from callus was significantly improved by 6-benzyladenine (BA), and a mean number of 48.4 PLBs was produced from 100 mg calli at 1.0 mg l(-1) BA within 3 months. BA and thidiazuron (TDZ) promoted the direct formation of PLBs from explants. The highest frequency of direct PLBs formation (76.0 %) and the highest mean number of PLBs per explant (30.2) were observed in protocorms cultured with 0.5 mg l(-1) BA. Assessment of clonal fidelity by inter-simple sequence repeat (ISSR) markers revealed similarity ranges of 99.8-100.0 % between the regenerants and their mother plants and 99.5-100.0 % among the regenerants, which suggested the micropropagation protocols were genetically stable.