文献类型：期刊文献

中文题名：淡紫拟青霉液体发酵条件的优化

英文题名：Optimal Cultivation for Liquid Fermentation of Paecilomyces lilacinus

作者：王曦茁[1] 刘春秀[1] 汪来发[1] 苟大平[1] 余明忠[2]

第一作者：王曦茁

机构：[1]中国林业科学研究院森林生态与保护研究所国家林业局森林保护学重点实验室;[2]四川省茂县林业局

年份：2012

卷号：30

期号：4

起止页码：410-414

中文期刊名：四川农业大学学报

外文期刊名：Journal of Sichuan Agricultural University

收录：北大核心:【北大核心2011】；CSCD:【CSCD_E2011_2012】；

基金：科技部农业科技成果转化资金项目(2010GB24320615);国家自然科学基金青年基金(31100477)

语种：中文

中文关键词：淡紫拟青霉;液体发酵;条件筛选

外文关键词：Paecilomyces lilacinus; liquid fermentation; screening of fermentation conditions

分类号：Q939.96;Q476.1

摘要：【目的】对食线虫真菌淡紫拟青霉液体培养条件进行优化,从而获得优化的淡紫拟青霉液体发酵条件。【方法】研究淡紫拟青霉在不同碳源、氮源、无机盐、初始pH值下对产孢量的影响,确定了适宜的培养条件及发酵培养时间。【结果】最佳基础培养基配方为A1(g/L):葡萄糖40,NaNO320,KH2PO40.5,MgSO4.7H2O 0.5;最佳复合培养基配方为b(g/L):葡萄糖40,NaNO310,(NH4)2SO45,黄豆粉5,KH2PO40.5,MgSO4.7H2O 0.5;其他培养条件的选择结果:淡紫拟青霉最适产孢的初始pH值为6.0～7.0,发酵温度26℃,发酵培养时间为72h到80h。【结论】通过这些研究获得了淡紫拟青霉液体优化的发酵条件,为该菌大规模产生奠定基础。
[Objective] Our aim was to optimize the culture conditions of Paecilomyces lilacinus liquid of enmatophagous fungus, and to obtain the optimal conditions of liquid fermentation of P. lilacinus. [Method] P. lilacinus liquid was cultured under different carbon sources, nitrogen sources, inorganic salts, pH values and times. The amount of spores in each treatment was meas ured. Optimal condition and time were selected. [Results] @ The optimal basic medium A1 （g/ L）.. glucose 40, NaNO3 20, KH2PO4 0.5, MgSO4 · 7H20 0.5; （2） The optimal compound medi- um b （g/L）. glucose 40, NaNO310, （NH4）2SO4 5, soybean powder 5, KH2PO4 0.5, MgSO4· 7H20 0.5; ③ The best initial pH value was 6.0～7.0 and temperature was 26 ℃ ; ④ The opti- mum time for fermentation was 72～80 h. [Conclusion] This study optimizes the culture condi tions of P. lilacinus and provides foundation for large scale production of P. lilacinus.