文献类型：期刊文献

中文题名：崖柏和侧柏幼苗对自然降温的生理生化反应

英文题名：Physiological and Biochemical Responses of Thuja sutchuenensis and Platycladus orientalis Seedlings to Natural Cooling

作者：朱莉[1,2] 郭泉水[2] 金江群[2] 刘建锋[3] 李志勇[1] 颜华[4]

第一作者：朱莉

机构：[1]河南科技大学农学院,河南洛阳471003;[2]中国林业科学研究院森林生态环境与保护研究所,国家林业局森林生态环境重点实验室,北京100091;[3]中国林业科学研究院林业研究所,北京100091;[4]重庆市开县林业局,重庆开县404500

年份：2013

卷号：26

期号：2

起止页码：220-226

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：国家林业局野生动植物保护专项:“世界级极危物种崖柏潜在分布和生态适应性研究(2012-1)”;“珍稀濒危物种崖柏野外救护与人工繁育(优化项目)(1691000401)”;中央公益性科研院所专项基金(RIF2010-15)

语种：中文

中文关键词：崖柏;侧柏;自然降温;生理生化;反应

外文关键词：Thuja sutchuenensis; Platycladus orientalis; seasonal cooling; plant physiology; plant biochemistry; re-sponse

分类号：S792.360.1

摘要：以崖柏和侧柏幼苗为对象,研究其叶片在秋冬季节性自然降温过程中抗氧化酶、渗透调节物质和丙二醛(MDA)的变化特征,并比较两树种对低温胁迫的适应能力。结果表明:在秋冬季节性自然降温过程中,日最低气温由12.50℃降至-13.96℃,崖柏幼苗叶片的超氧化物歧化酶(SOD)活性均显著增强,但过氧化物酶(POD)活性基本没有变化,而侧柏的SOD和POD活性同时增强;两树种叶片脯氨酸(Pro)含量、可溶性糖含量均显著增加,但崖柏的增加幅度低于侧柏;两树种叶片MDA含量均极显著增加,但崖柏增加幅度较大。日最低气温与侧柏的SOD、POD和可溶性糖含量均极显著相关,但仅与崖柏的SOD极显著相关,表明在季节性自然降温过程中,侧柏比崖柏更能够建立较完善的防御系统进行自身调节以适应低温环境。研究结果可为崖柏迁地保存和栽培管理工作提供参考。
The paper focused on two species seedlings, Thuja sutchuenensis and Platycladus orientalis. Their adapt-ability to low temperature stress was compared by detecting and analyzing the leaves variable characteristics of an-tioxidant enzymes, osmotic adaptive substances and malondialdehyde （MDA） in the process of seasonal cooling in autumn and winter. The results showed that ： during the process of seasonal cooling, the daily minimum air tempera- ture decreased from 12. 5 ℃ to - 13.96 ℃, the superoxide dismutase （SOD） activity of T. sutchuenensis was strengthened significantly, however, the change of Peroxidase （POD） activity was not obvious, and both SOD and POD activities of P. orientalis were strengthened. The content of proline （Pro） and soluble sugars in leaves in-creased significantly in two species, T. sutchuenensis was lower than P. orientalis. The MDA content in leaves of the two species increased significantly, while T. sutchuenensis increased more than P. orientalis. The daily mini-mum air temperature was significantly correlated with the SOD, POD activity and soluble sugar content in P. orien-talis, but it just correlated with the SOD activity in T. sutchuenensis. All these suggest that, during seasonal cool-ing, P. orientalis is more adaptive to low temperature stress than T. sutchuenensis through establishing better defen-sive system and conducting self-adjustment. The results of the research can provide reference for the T. sutchuenen-s/s ex-situ conservation and cultivation management.