文献类型：期刊文献

英文题名：Genetic diversity and relationship of endangered plant Magnolia officinalis (Magnoliaceae) assessed with ISSR polymorphisms

作者：Yu, Hua-Hui[1] Yang, Zhi-Ling[1] Sun, Bo[2] Liu, Ruo-nan[1]

通信作者：Yang, ZL[1]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Fuyang City 311400, Zhejiang, Peoples R China;[2]Danghu High Sch, Pinghu City 314200, Zhejiang, Peoples R China

年份：2011

卷号：39

期号：2

起止页码：71-78

外文期刊名：BIOCHEMICAL SYSTEMATICS AND ECOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000289611200001)】；

基金：We thank forestry bureau of every county for their great help in collecting the samples. This work was supported by the Ministry of Science and Technology of the People's Republic of China (No. 200704022) and Major Program of Natural Science Foundation of Zhejiang Province (No. Z3100041).

语种：英文

外文关键词：ISSR; Magnolia officinalis; Genetic diversity; Genetic relationship

摘要：Twenty-eight populations of the rare medicinal plant Magnolia officinalis (Magnoliaceae) were sampled across its natural range, and inter-simple sequence repeats (ISSRs) markers were used to assess the genetic variation within and among populations. Twelve primer combinations produced a total of 137 unambiguous bands of which 114 (83.2%) were polymorphic. M. officinalis exhibited a relatively low genetic diversity at population level (the percentage of polymorphic loci PPB = 49.8%. Nei's genetic diversity H = 0.194, Shannon's information index I = 0.286). However, the genetic diversity at species level was relatively high (PPB = 83.2%; H 0.342; I = 0.496). The coefficient of gene differentiation (G(ST), 42.8%) and the results of analysis of molecular variance (AMVOA) indicated that genetic differentiation occurred mainly within populations. The estimated gene flow (Nm) from G(ST) was 0.669. It indicated that the fragmentation and isolation of populations might result from specific evolutionary history and anthropogenic activity. Genetic drift played a more important role than gene flow in the current population genetic structure of M. officinalis. Conservation strategies for this rare species are proposed based on the genetic data. (C) 2010 Elsevier Ltd. All rights reserved.