文献类型：期刊文献

英文题名：SOMATIC EMBRYOGENESIS AND PLANT REGENERATION FROM CULTURED IMMATURE EMBRYOS OF CATALPA BUNGEI C. A. MEY

作者：Liang, Hongwei[1] Wang, Yuyu[1] Cen, Yunxin[1] Wang, Changlan[1] Yang, Guijuan[2] Chen, Faju[1]

第一作者：Liang, Hongwei

通信作者：Chen, FJ[1]

机构：[1]China Three Gorges Univ, Key Lab Three Gorges Reg Plant Genet & Germplasm, Biotechnol Res Ctr, 8 Univ Rd, Yichang 443002, Hubei, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, 1 Dong Xiaofu, Beijing 100091, Peoples R China

年份：2020

卷号：20

期号：2

起止页码：48-56

外文期刊名：PROPAGATION OF ORNAMENTAL PLANTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000582335200002)】；

基金：This research was supported by grants from the National Science and Technology Planning Project of "12th Five-Year" in Rural Areas (Grant No.2012BAD21 B0303) and the 13th five-year National Key Research and Development Program (Grant No. 2017YFD0600604).

语种：英文

外文关键词：callus; plant growth regulators; plant regeneration; somatic embryos

摘要：Catalpa bungei is an ornamental tree and high-quality timber species unique to China. Herein. Immature zygotic embryos of C. bungei were used as explants to investigate the effects of different concentrations and combinations of plant growth regulators (PGRs) on somatic embryogenesis capacity and plant regeneration. After 15 days, the highest frequency of initial calluses development (90%) was obtained on half-strength MS medium supplemented with 1.0 mg l(-1) 2,4-Dichlorophenoxyacetic acid (2,4-D) and 0.1 mg l(-1) N-6-benzyladenine (BA). The initial calluses turned from white to black and compact granular calluses when they were transferred for proliferation in the half-strength MS medium containing 10 mg l(-1) BA and 0.01 mg l(-1) alpha-naphthaleneacetic acid (NAA). A light yellow embryogenic callus could be induced from the dark colored one during induction of somatic embryogenesis. The highest frequency (59.8%) of somatic embryos induction was achieved on half-strength MS medium supplemented with 0.2 mg l(-1) BA and 0.05 mg l(-1) NAA after 2.5 months subcultivation. They subsequently converted into globular-, heart-, torpedo-, and cotyledonary-stage somatic embryos being subcultured on the same medium. Cotyledonary-stage embryos were transferred on a PGRs-free plant regeneration medium and finally developed into complete plantlets after 40 days' subcultivation. The rooting rate reached to 92.3% when the stems derived from somatic seedlings were cultured at the half-strength MS medium supplemented 1.0 mg l(-1) IBA. Based on the histological analysis, the development of somatic embryos was similar to that of zygotic embryos. Starch accumulation occurred only during the induction of embryogenic callus from initial callus, which was closely related to the energy supply of somatic embryogenesis.