文献类型：期刊文献

英文题名：Integrated analysis of multiomic data reveals the role of the antioxidant network in the quality of sea buckthorn berry

作者：He, Caiyun[1] Zhang, Guoyun[1] Zhang, Jianguo[1,2] Zeng, Yanfei[1] Liu, Juanjuan[1]

第一作者：何彩云

通信作者：Zhang, JG[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat, State Forestry Adm,Res Inst Forestry, Beijing, Peoples R China;[2]Nanjing Forestry Univ, Collaborat Innovat Ctr Sustainable Forestry South, Nanjing, Jiangsu, Peoples R China

年份：2017

卷号：31

期号：5

起止页码：1929-1938

外文期刊名：FASEB JOURNAL

收录：;Scopus(收录号：2-s2.0-85019421478);WOS:【SCI-EXPANDED(收录号:WOS:000399195500015)】；

基金：The authors thank Hongmei Luo (Chinese Academy of Forestry) for helping with the sampling. This work was supported by the Basic Research Fund of Rapid Innovation Fund (RIF; Grant RIF-2014-04), National Natural Science Foundation of China (31470616), and the Special Fund for Forest Scientific Research in the Public Welfare (201504103). RNA-seq data have been deposited at the European Nucleotide Archive under accession PRJEB12839. MS proteomics data have been deposited to the ProteomeXchange Consortium via the Proteomics Identifications (PRIDE) partner repository with data set identifier PXD004251. The authors declare no conflicts of interest.

语种：英文

外文关键词：ascorbic acid; berry colorcarotenoids; flavonoids; UDP-glucuronosyltransferase

摘要：Berries of sea buckthorn, known as the "king of vitamin C," are abundant in antioxidants, have attractive colors, and are an excellent material with which to study the relationships between berry color, antioxidants, and berry quality. No study has yet determined the molecular basis of the relationship between sea buckhorn berries and their color and antioxidant levels. By using RNA-seq, LC-MS/MS, and LC/GC-MS technology and selecting red (darkest colored) and yellow (lightest colored) sea buckthorn berries at different development stages, this study showed that the red and yellow berry resulted from a higher ratio of lycopene to beta-carotene and of b-carotene to lycopene content, respectively. The uronic acid pathway-a known animal pathway-in ascorbic acid synthesis was found in sea buckthorn berries, and the higher expression of UDP-glucuronosyltransferase in red berries was consistent with the higher content of ascorbic acid. In summary, multiomic data showed that the color of sea buckthorn berries is mainly determined by b-carotene and lycopene; red sea buckthorn berries were richer than yellow berries in antioxidants, such as carotenoids, flavonoids, and ascorbic acid; and the animal pathway might be operating in sea buckthorn.-He, C., Zhang, G., Zhang, J., Zeng, Y., Liu, J. Integrated analysis of multiomic data reveals the role of the antioxidant network in the quality of sea buckthorn berry.