文献类型：期刊文献

英文题名：PagGRF12a interacts with PagGIF1b to regulate secondary xylem development through modulating PagXND1a expression in Populus alba x P. glandulosa

作者：Wang, Jinnan[1,2] Zhou, Houjun[1,3] Zhao, Yanqiu[1,4] Jiang, Cheng[1,4] Li, Jihong[2] Tang, Fang[1,5] Liu, Yingli[1] Zhao, Shutang[1,5] Hu, Jianjun[1,5] Song, Xueqin[1,5] Lu, Meng-Zhu[1,4,5]

第一作者：Wang, Jinnan

通信作者：Song, XQ[1];Song, XQ[2]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat State Forestry A, Beijing 100091, Peoples R China;[2]Shandong Agr Univ, Coll Forestry, Key Lab Agr Ecol & Environm, Tai An 271018, Shandong, Peoples R China;[3]Ludong Univ, Sch Agr, Yantai 264025, Peoples R China;[4]Zhejiang Agr & Forestry Univ, Sch Forestry & Biotechnol, State Key Lab Subtrop Silviculture, Hangzhou 311300, Peoples R China;[5]Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, Nanjing 210037, Peoples R China

年份：2021

卷号：63

期号：10

起止页码：1683-1694

外文期刊名：JOURNAL OF INTEGRATIVE PLANT BIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000679940100001)】；

基金：This work was supported by the National Natural Science Foundation of China (31570676), the National Key Program on Transgenic Research of China (2018ZX08020-002), the Basic Research Fund of Research Institute of Forest, Chinese Academy of Forestry (RIF-2014-08), the National Key Research and Development Program of China (2016YFD0600103), the National Key Program on Transgenic Research of China (2018ZX08020-002), and the Ten-thousand Talents Program of China for Meng-Zhu Lu.

语种：英文

外文关键词：GRF; GIF1; Populus; wood formation; XND1; xylem development

摘要：Growth-regulating factors (GRFs) are important regulators of plant development and growth, but their possible roles in xylem development in woody plants remain unclear. Here, we report that Populus alba x Papulus glandulosa PagGRF12a negatively regulates xylem development in poplar. PagGRF12a is expressed in vascular tissues. Compared to non-transgenic control plants, transgenic poplar plants overexpressing PagGRF12a exhibited reduced xylem width and plants with repressed expression of PagGRF12a exhibited increased xylem width. Xylem NAC domain 1 (XND1) encodes a NAC domain transcription factor that regulates xylem development and transcriptional analyses revealed that PagXND1a is highly upregulated in PagGRF12a-overexpressing plants and downregulated in PagGRF12a-suppressed plants, indicating that PagGRF12a may regulate xylem development through PagXND1a. Transient transcriptional assays and chromatin immunoprecipitation-polymerase chain reaction assays confirmed that PagGRF12a directly upregulates PagXND1a. In addition, PagGRF12a interacts with the GRF-Interacting Factor (GIF) PagGIF1b, and this interaction enhances the effects of PagGRF12a on PagXND1a. Our results indicate that PagGRF12a inhibits xylem development by upregulating the expression of PagXND1a.