文献类型：期刊文献

中文题名：檀香SaMYB36基因克隆及原核表达分析

英文题名：Gene Cloning and Prokaryotic Expression Analysis of SaMYB36 Gene in Santalum album

作者：周云清[1,2] 杨舒淇[1] 陆俊锟[1] 胡立盼[2] 覃方锉[1] 孟森[1]

第一作者：周云清

机构：[1]中国林业科学研究院热带林业研究所,广东广州510520;[2]重庆三峡学院生物与食品工程学院,重庆404100

年份：2025

卷号：40

期号：6

起止页码：120-127

中文期刊名：西北林学院学报

外文期刊名：Journal of Northwest Forestry University

收录：;北大核心:【北大核心2023】；

基金：广州市基础与应用基础研究专题(SL2022A04J00577);重庆市自然科学基金项目(CSTB2023NSCQ-MSX0021)。

语种：中文

中文关键词：檀香;SaMYB36;干旱胁迫;基因克隆;原核表达分析

外文关键词：Santalum album;SaMYB36;drought stress;gene cloning;prokaryotic expression analysis

分类号：S792.99

摘要：MYB转录因子在萜类化合物的生物合成中发挥重要作用。通过酵母文库筛选得到1个可能与檀香萜合成酶基因启动子相互作用的候选基因SaMYB36。本研究从檀香中克隆SaMYB36基因,对其进行生物信息学、蛋白亚细胞定位、组织表达、干旱胁迫表达和原核表达分析,为进一步探究其在干旱诱导檀香心材形成过程中的功能奠定理论基础。结果显示,SaMYB36基因开放阅读框全长744 bp,预测编码蛋白分子量为26.97 ku;SaMYB36蛋白含有典型的R2R3-MYB结构域,属于R2R3-MYB型转录因子。系统发育分析结果显示,SaMYB36蛋白与MtMYB36和TwMYB36-like蛋白的亲缘关系较近。亚细胞定位表明,SaMYB36蛋白定位在细胞核。组织特异性分析表明,SaMYB36基因在檀香过渡区中显著富集表达。干旱胁迫处理的3和9 d,檀香中SaMYB36基因表达水平显著升高,表明SaMYB36基因对干旱响应明显。构建重组pET-SUMO-SaMYB36表达载体,在大肠杆菌中成功表达并纯化得到SaMYB36蛋白。
The MYB transcription factor serves as a pivotal regulator in the biosynthetic pathway of terpenoids.Utilizing yeast library screening methodology,we isolated a promising candidate gene,SaMYB36,which potentially interacts with the promoter region of the santalene synthase gene.To elucidate the functional role of SaMYB36 in sandalwood,a comprehensive analysis was undertaken encompassing gene cloning,bioinformatics characterization,subcellular localization of the SaMYB36 protein,tissue-specific and drought stress-responsive expression profiling,and its prokaryotic expression,to lay the theoretical groundwork for further investigations into the mechanisms underpinning the role of SaMYB36 in drought-induced heartwood formation in sandalwood.The results revealed that the total length of the gene was 744 bp and the predicted molecular weight of the encoded protein was 26.97 ku.Notably,SaMYB36 harbors the canonical R2R3-MYB domain,classifying it as a R2R3-MYB transcription factor family member.Evolutionary analysis showed that SaMYB36 was closely related to MtMYB36 and TwMYB36-like proteins.Furthermore,subcellular localization studies confirmed the nuclear localization of the SaMYB36 protein.Tissue-specific expression analysis highlighted the preferential enrichment and expression of SaMYB36 in the transition zone of sandalwood.Under drought stress conditions,the expression levels of SaMYB36 were significantly upregulated at 3,and 9 days post-treatment,suggesting its sensitivity and responsiveness to drought stimuli.The successful construction of a recombinant pET-SUMO-SaMYB36 expression vector facilitated its expression in Escherichia coli.