文献类型：期刊文献

英文题名：Enzyme-assisted deep eutectic solvent extraction of Litsea cubeba essential oil and its anti- Aspergillus flavus activity

作者：Zhang, Jiantao[1,2] Tao, Ran[3] Wang, Yangdong[1,2] Jiang, Jianchun[3] Chen, Yicun[1,2]

第一作者：Zhang, Jiantao

通信作者：Chen, YC[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Peoples R China;[3]CAF, Inst Chem Ind Forest Prod, Nanjing 210042, Jiangsu, Peoples R China

年份：2025

卷号：217

外文期刊名：LWT-FOOD SCIENCE AND TECHNOLOGY

收录：;EI(收录号：20250417744128);Scopus(收录号：2-s2.0-85215582045);WOS:【SCI-EXPANDED(收录号:WOS:001409989000001)】；

基金：This project was supported by the National Nonprofit Institute Research Grant of CAFINT (No. AFYBB2022ZA002) and the Ten Thou-sand People Plan of Science and Technology Innovation Leading Talent of Zhejiang, China (No. 2022R52028) awarded to Yicun Chen.

语种：英文

外文关键词：Enzyme-assisted; Deep eutectic solvent extraction; Essential oil; Antifungal activity; Green chemistry

摘要：The essential oil of Litsea cubeba demonstrates notable antibacterial properties and holds considerable promise for various applications. To explore green and effective extraction method for L. cubeba essential oil, we introduced enzyme-assisted natural deep eutectic solvents (DESs). The optimization of DESs was performed using response surface methodology with a Box-Behnken design (BBD), and the optimal parameters were determined as follows: a choline chloride to citric acid (ChCl/CA) ratio of 1:0.97, water content of 73%, and cellulase (0.5%) enzymolysis temperature of 45 degrees C. Compared to traditional steam distillation, the DESs method significantly increased the essential oil yield from 3.20% to 4.70% and expanded the chemical profile of the essential oil, identifying a total of 79 compounds. Five new aromatic compounds, including methyl geranate, geranic acid, pmentha-1,5-dien-8-ol, alpha-terpinyl acetate, and terpinyl acetate, were identified. Antifungal activity against Aspergillus flavus was confirmed by scanning electron microscopy. At a concentration of 1000 mu L/L, after 96 h of incubation, essential oils extracted using enzyme-assisted DESs method demonstrated substantial inhibition against A. flavus, with the ChCl/EG formulation achieving a 100% inhibition rate. The content of citral, citronellal, citronellol, linalool, and new compound methyl geranate showed a positive correlation with the anti- A. flavus inhibition rate. The enzyme-assisted DESs extraction method proves to be an effective and efficient approach for extracting L. cubeba essential oil and provides a valuable reference for future research and application in essential oil extraction.