文献类型：期刊文献

中文题名：山茶WRKY基因家族鉴定及其在花色形成中的表达分析

英文题名：Identification and expression analysis of WRKY gene family in flower color formation in Camellia japonica

作者：宋志欣[1,2] 范梦龙[1] 姜泓[1] 张莹[1] 李辛雷[1]

第一作者：宋志欣

机构：[1]中国林业科学研究院亚热带林业研究所,浙江杭州311400;[2]南京林业大学,江苏南京210037

年份：2025

卷号：45

期号：7

起止页码：188-196

中文期刊名：中南林业科技大学学报

外文期刊名：Journal of Central South University of Forestry & Technology

收录：;北大核心:【北大核心2023】；

基金：浙江省农业(花卉新品种选育)新品种选育重大科技专项(2021C02071-2)。

语种：中文

中文关键词：山茶;WRKY基因家族;花色;表达分析

外文关键词：Camellia japonica;WRKY gene family;flower color;expression analysis

分类号：S794.9

摘要：【目的】WRKY基因家族对植物生长发育具有重要调控作用,山茶是重要的观赏植物,花色丰富,鉴定分析山茶WRKY基因家族成员并探讨其在不同颜色山茶花瓣中的表达模式具有重要意义。【方法】基于山茶全基因组数据,采用生物信息学方法,对山茶WRKY基因家族进行鉴定分析,并基于山茶花色相关转录组数据,分析CjWRKYs在不同颜色山茶花瓣中的表达模式。【结果】在山茶全基因组中鉴定出94个WRKY基因家族成员,命名为CjWRKY1~CjWRKY94;系统发育树将其分为Ⅰ、Ⅱ和Ⅲ 3组,Ⅱ组又分为Ⅱ-a、Ⅱ-b、Ⅱ-c、Ⅱ-d和Ⅱ-e5个亚群;Ⅰ组成员含有2个WRKY结构域,Ⅱ组和Ⅲ组成员仅含有1个WRKY结构域;共鉴定出10个保守基序和5个保守结构域,同一亚群内CjWRKYs成员具有相似的保守基序、保守结构域和基因结构;CjWRKYs启动子区域含有光响应、胁迫、激素响应等38种作用元件,包括调控类黄酮生物合成的MYB结合位点;94个CjWRKYs不均匀分布于15条染色体上,存在13对基因串联重复事件;共线性分析表明,94个CjWRKYs基因中存在72个片段复制事件,片段复制基因Ka/Ks值均小于1;与拟南芥WRKY基因家族存在116个共线基因对;CjWRKYs在不同山茶花色中的表达谱分析结果显示,87个CjWRKYs呈现表达差异,其中32个CjWRKYs随山茶花瓣颜色加深显著上调表达,6个CjWRKYs下调表达,推测这些CjWRKYs在山茶花青苷合成中起重要调控作用;推测CjWRKY48、CjWRKY53和CjWRKY66通过与MYB转录因子互作正向调控花青苷合成,进而影响山茶花色。【结论】在山茶全基因组中鉴定出94个CjWRKYs基因,大多数CjWRKYs参与山茶花青苷合成,在山茶花色形成中发挥重要作用。
【Objective】The WRKY gene family plays an important role in the regulation of plant growth and development.Camellia japonica is an important ornamental plant with rich flower colors.It is of great significance to identify and analyze the WRKY gene family members in Camellia japonica petals with different colors.【Method】Based on the whole genome data of Camellia japonica,the WRKY gene family was identified and analyzed by bioinformatics methods.Based on the transcriptome data related to camellia flower color,the expression pattern of CjWRKYs in different colored camellia petals was analyzed.【Result】The results indicated that 94 CjWRKY genes were identified in the whole genome and named CjWRKY1-CjWRKY94.The phylogenetic tree divided the strains into three groups(I,II and III),and group II was further divided into five subgroups(II-a,II-b,II-c,II-d and II-e).Group I members contained two WRKY domains,while group II and III members contained only one WRKY domain.A total of 10 conserved motifs and 5 conserved domains were identified.The CjWRKYs members in the same subgroup had similar conserved motifs,conserved domains and gene structures.The CjWRKYs promoter region contained 38 elements for light response,stress response and hormone response,including MYB binding site for regulating flavonoid biosynthesis.94 CjWRKYs were unevenly distributed on 15 chromosomes,and there were 13 pairs of tandem duplication events.Collinearity analysis showed that there were 72 duplication events in 94 CjWRKYs genes,and the Ka/Ks value of the duplication genes was less than 1.There were 116 collinear gene pairs with Arabidopsis WRKY gene family.Among them,32 CjWRKYs were up-regulated and 6 CjWRKYs were down-regulated with the deepening of flower color of Camellia japonica,which suggested that CjWRKYs played an important role in the regulation of anthocyanin synthesis.It is speculated that CjWRKY48,CjWRKY53 and CjWRKY66 positively regulate anthocyanin synthesis by interacting with MYB transcription factors,thereby affecting flower color of Camellia japonica.【Conclusion】94 CjWRKYs genes were identified in the whole Camellia japonica genome,and most CjWRKYs are involved in anthocyanin synthesis and play an important role in color formation of Camellia japonica.