文献类型：期刊文献

中文题名：卡特兰ChCHS1和ChDFR1基因的克隆及表达分析

英文题名：Cloning and Expression Analysis of ChCHS1 and ChDFR1 Genes From Cattleya

作者：王紫珊[1] 周琳[1] 王雁[1]

机构：[1]中国林业科学研究院林业所国家林业局林木培育重点实验室

年份：2015

卷号：36

期号：7

起止页码：1280-1289

中文期刊名：热带作物学报

外文期刊名：Chinese Journal of Tropical Crops

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD_E2015_2016】；

基金：国家林业局948项目

语种：中文

中文关键词：卡特兰;查尔酮合酶基因;二氢黄酮醇4-还原酶基因;克隆;表达分析

外文关键词：Cattleya hybrida; Chalcone synthase gene; Dihydroflavonol 4-reductase gene; Cloning; Expression analysis

分类号：S682.3

摘要：以卡特兰紫色花品种‘粉女郎’(Cattleya hybrid‘Pink Lady’)为材料,采用RT-PCR和RACE方法从花瓣中分离得到了一个查尔酮合酶(chalcone synthase,CHS)和一个二氢黄酮醇4-还原酶(dihydroflavonol 4-reductase,DFR)同源基因的cDNA全长,分别命名为ChCHS1和ChDFR1,GenBank登录号分别为KP171693和KP171694。序列分析结果发现:Ch CHS1的cDNA全长1 508 bp,编码394个氨基酸;Ch DFR1基因的c DNA全长1 250 bp,编码350个氨基酸。同源性检索和生物信息学分析表明,这2个基因编码的蛋白都具有各自的功能位点和保守性特征多肽序列。氨基酸序列比对与系统进化树分析显示,ChCHS1、ChDFR1基因在兰科中与蕙兰、石斛兰关系最近,与百合科关系较近。相对实时荧光定量PCR结果表明,ChCHS1和ChDFR1都在蕾期表达量最低,伴随花朵的开放,表达量逐渐上升,最终分别在花朵盛开期和接近开放时达到最高。
The full-length cDNA sequences of chalcone synthase （CHS） and dihydroflavonol 4-reductase （DFR） genes were cloned from Cattleya hybrida ＇Pink lady＇ by using RT-PCR and RACE, named ChCHS1 and ChDFR1. The GenBank accession was No. KP171693 and No. KP171694 recpectively. The bioinformatics analysis indicated that ChCHS1 is 1 508 bp in full length, encoding a polypeptide of 394 amino acids while ChDFR1 is 1 250 bp in full length, encoding a 350 predicted amino acids residues. The sequence and homology of GenBank revealed that CHS and DFR had the conserved active sites and the family signture. Sequence and phylogenetic analysis indicated a high degree homeotic between Cattleya and Cymbidium or Dendrobium, and in different family CHS and DFR shared more than 70% homology with Liliaceae. Relative real-time PCR analysis showed that the highest expression of ChCHS1 was when flower was in bloom. When the flower was nearly open, ChDFR1 had a highest abundant transcript. In bud stage, ChCHS1 and ChDFR1 had the low level expression.