文献类型：期刊文献

中文题名：光臀八齿小蠹共生微生物群落组成及其功能基因注释

英文题名：Community composition and functional gene annotation of the symbiotic microbes in Ips nitidus(Coleoptera:Scolytinae)

作者：刘彩霞[1] 李刚[2] 梁玲瑜[1] 韩富忠[3] 王正[4] 王慧敏[1] 吕全[1]

第一作者：刘彩霞

机构：[1]中国林业科学研究院森林生态环境与自然保护研究所,国家林业和草原局森林保护学重点实验室,北京100091;[2]内蒙古自治区巴彦淖尔市乌拉特前旗森林病虫害防治检疫站,巴彦淖尔015006;[3]青海省黄南州麦秀林场,黄南811300;[4]山东农业大学植物保护学院,泰安271018

年份：2023

卷号：66

期号：10

起止页码：1329-1342

中文期刊名：昆虫学报

外文期刊名：Acta Entomologica Sinica

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD2023_2024】；

基金：国家自然科学基金项目(32071769,31770682)。

语种：中文

中文关键词：光臀八齿小蠹;共生菌;功能基因;相互作用;萜烯类降解;长喙壳类真菌

外文关键词：Ips nitidus;symbionts;functional gene;interaction;terpenoid degradation;ophiostomatoid fungi

分类号：S763

摘要：【目的】揭示光臀八齿小蠹Ips nitidus共生微生物群落组成和功能特征。【方法】对来自中国西北部青海省麦秀自然保护区的光臀八齿小蠹进行肠道和其他组织共生真菌和细菌宏基因组学和相关性网络分析,比较光臀八齿小蠹肠道和其他组织共生真菌及细菌群落组成,分析微生物群落间的相互关系,并采用非冗余蛋白序列比对方法注释共生微生物功能基因。【结果】共发现光臀八齿小蠹肠道和其他组织共生微生物3520种,真菌和细菌的物种数最高,分别占全部物种数的23.21%和69.01%,平均相对丰度分别为9.69%和13.79%。其他组织中共生真菌白粉菌目(Erysiphales)相对丰度和物种多样性较肠道中的高,而其他组织中酵母目(Saccharomycetales)相对丰度和物种多样性较肠道中的低;其他组织中长喙壳类真菌(ophiostomatoid fungi)多样性(6属16种)较在肠道中的(5属13种)高,在其他组织和肠道中的平均相对丰度分别为0.016%和0.013%。其他组织中肠杆菌目(Enterobacterales)相对丰度和物种多样性高于肠道中的。肠杆菌属Enterobacter、白粉菌属Erysiphe、丛枝菌根属Rhizophagus和沃尔巴克氏菌属Wolbachia均为肠道和其他组织的核心菌属。真菌和细菌的某些群落在肠道和其他组织间表现出相反的相互关系,表明光臀八齿小蠹共生菌间可能存在复杂的相互作用。分别鉴定到共生真菌和细菌功能基因7010和6483个,细菌中异源性物质生物降解和代谢相关功能基因数量远高于真菌的;mRNA监测通路、丙酸代谢和泛素介导的蛋白质水解等11条通路在肠道中较在其他组织中具有更多的差异基因;对于柠檬烯降解和甾醇合成相关功能基因的注释结果表明,细菌对柠檬烯较真菌具有明显的降解能力,而真菌在甾醇生物合成中较细菌发挥更重要的促进作用;74个长喙壳类真菌功能基因主要参与到氧化磷酸化、代谢通路和氨基酸代谢,且长喙壳类真菌在其他组织中多样性更高,主要分布于Ophiostoma等。【结论】光臀八齿小蠹肠道和其他组织中共生微生物的群落组成和多样性以及功能上存在一定的差异性,肠杆菌属、白粉菌属、丛枝菌根属和沃尔巴克氏菌属是光臀八齿小蠹共生微生物群落中最重要的核心菌群,可能在群落构建中发挥着重要的作用。
【Aim】To reveal the community composition and functional characteristics of the symbiotic microbes in Ips nitidus.【Methods】Metagenomics and correlation network analysis were conducted on the symbiotic fungi and bacteria in the gut and other tissues of I.nitidus from Maixiu Nature Reserve in Qinghai Province,Northwest China to compare the community composition of the symbiotic fungi and bacteria in the gut and other tissues of I.nitidus,to analyze the interrelationships of the microbial communities and to annotate the functional genes of the symbiotic microbes by non-redundant protein sequence alignment method.【Results】A total of 3520 species of symbiotic microbes were found in the gut and other tissues of I.nitidus.Fungi and bacteria possessed the highest numbers of species(accounting for 23.21%and 69.01%of all species,respectively),with the average relative abundance of 9.69%and 13.79%,respectively.For fungal symbionts,Erysiphales showed higher relative abundance and species diversity in other tissues than in the gut,and Saccharomycetales showed lower relative abundance and species diversity in other tissues than in the gut.Ophiostomatoid fungi in other tissues showed higher species diversity than those in the gut(16 species of 6 genera,and 13 species of 5 genera,respectively).The average relative abundance of ophiostomatoid fungi in other tissues and gut occupied 0.016%and 0.013%,respectively.The relative abundance and species diversity of Enterobacterales in other tissues were higher than those in the gut.Enterobacter,Erysiphe,Rhizophagus and Wolbachia were the core genera in the gut and other tissues.Some communities of fungi and bacteria showed opposite interrelationships in the gut and other tissues,suggesting that there may be complex interactions between the symbionts in I.nitidus.A total of 7010 and 6483 functional genes were identified from symbiotic fungi and bacteria,respectively.The number of functional genes related to xenobiotics biodegradation and metabolism in bacteria was higher than that in fungi.However,11 pathways such as mRNA surveillance pathway,propanoate metabolism,ubiquitin-mediated proteolysis and so on had more significant differential genes in the gut than in other tissues.The annotation results for functional genes related to limonene degradation and sterol synthesis showed that bacteria may degrade limonene significantly compared with fungi,but fungi may play a more crucial role in promoting sterol biosynthesis than bacteria.A total of 74 functional genes of ophiostomatoid fungi are mainly involved in oxidative phosphorylation,metabolic pathway and amino acid metabolism.Moreover,ophiostomatoid fungi were of higher diversity in other tissues and mainly distributed in Ophiostoma,etc.【Conclusion】There are some differences in the community composition,diversity and function of the symbiotic microbes in the gut and other tissues of I.nitidus.Enterobacter,Erysiphe,Rhizophagus and Wolbachia are the most important core flora,and they might play an important role in the community assembly.