文献类型：期刊文献

英文题名：Salt-Sensitive Signaling Networks in the Mediation of K+/Na+ Homeostasis Gene Expression in Glycyrrhiza uralensis Roots

作者：Lang, Tao[1] Deng, Shurong[2,3] Zhao, Nan[2] Deng, Chen[2] Zhang, Yinan[2] Zhang, Yanli[2] Zhang, Huilong[2] Sa, Gang[2] Yao, Jun[2] Wu, Caiwu[4] Wu, Yanhong[1] Deng, Qun[1] Lin, Shanzhi[2] Xia, Jianxin[1] Chen, Shaoliang[2]

第一作者：Lang, Tao

通信作者：Xia, JX[1];Chen, SL[2]

机构：[1]Minzu Univ China, Coll Life & Environm Sci, Beijing, Peoples R China;[2]Beijing Forestry Univ, Coll Biol Sci & Technol, Beijing Adv Innovat Ctr Tree Breeding Mol Design, Beijing, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China;[4]Hebei Normal Univ Nationalities, Coll Resource & Environm Sci, Chengde, Peoples R China

年份：2017

卷号：8

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000407705300001)】；

基金：The research was supported jointly by the National Science-technology Support Plan Projects of China (2014BAC15B04), the National Natural Science Foundation of China (grant nos. 31570587 and 31270654), the Research Project of the Chinese Ministry of Education (grant no. 113013A), the Key Project for Oversea Scholars by the Ministry of Human Resources and Social Security of PR China (grant no. 2012001), the Program for Changjiang Scholars and Innovative Research Teams in University (grant no. IRT_17R08), and the Program of Introducing Talents of Discipline to Universities (111 Project, grant no. B13007).

语种：英文

外文关键词：liquorice; ion flux; eATP; H2O2; NO; NaCl; NMT; RT-qPCR

摘要：We investigated the effects of salt-sensitive signaling molecules on ionic fluxes and gene expression related to K+/Na+ homeostasis in a perennial herb, Glycyrrhiza uralensis, during short-term NaCl stress (100 mM, 24 h). Salt treatment caused more pronounced Na+ accumulation in root cells than in leaf cells. Na+ ions were mostly compartmentalized in vacuoles. Roots exposed to NaCl showed increased levels of extracellular ATP (eATP), cytosolic Ca2+, H2O2, and NO. Steady-state flux recordings revealed that these salt-sensitive signaling molecules enhanced NaCl-responsive Na+ efflux, due to the activated Na+/H+ antiport system in the plasma membrane (PM). Moreover, salt-elicited K+ efflux, which was mediated by depolarization-activated cation channels, was reduced with the addition of Ca2+, H2O2, NO, and eATP. The salt-adaptive effects of these molecules (Na+ extrusion and K+ maintenance) were reduced by pharmacological agents, including LaCl3 (a PM Ca2+ channel inhibitor), DMTU (a reactive oxygen species scavenger), cPTIO (an NO scavenger), or PPADS (an antagonist of animal PM purine P2 receptors). RT-qPCR data showed that the activation of the PM Na+/H+ antiport system in salinized roots most likely resulted from the upregulation of two genes, GuSOS1 and GuAHA, which encoded the PM Na+/H+ antiporter, salt overly sensitive 1 (SOS1), and H+-ATPase, respectively. Clear interactions occurred between these salt-sensitive agonists to accelerate transcription of salt-responsive signaling pathway genes in G. uralensis roots. For example, Ca2+, H2O2, NO, and eATP promoted transcription of GuSOS3 (salt overly sensitive 3) and/or GuCIPK (CBL-interacting protein kinase) to activate the predominant Ca2+-SOS signaling pathway in salinized liquorice roots. eATP, a novel player in the salt response of G. uralensis, increased the transcription of GuSOS3, GuCIPK, GuRbohD (respiratory burst oxidase homolog protein D), GuNIR (nitrate reductase), GuMAPK3, and GuMAPK6 (the mitogen-activated protein kinases 3 and 6). Moreover, GuMAPK3 and GuMAPK6 expression levels were enhanced by H2O2 in NaCl-stressed G. uralensis roots. Our results indicated that eATP triggered downstream components and interacted with Ca2+, H2O2, and NO signaling to maintain K+/Na+ homeostasis. We propose that a multiple signaling network regulated K+/Na+ homeostasis in NaCl-stressed G. uralensis roots.