文献类型：期刊文献

英文题名：Phenotypic and Comparative Transcriptome Analysis of Different Ploidy Plants in Dendrocalamus latiflorus Munro

作者：Qiao, Guirong[1,2] Liu, Mingying[1,2] Song, Kunlin[3] Li, Haiying[2] Yang, Huiqin[2] Yin, Yafang[3] Zhuo, Renying[1,2]

第一作者：乔桂荣

通信作者：Zhuo, RY[1];Zhuo, RY[2];Yin, YF[3]|[a00058eb5603bedf0c2e1]卓仁英;

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Key Lab Tree Breeding Zhejiang Prov, Hangzhou, Zhejiang, Peoples R China;[3]Chinese Acad Forestry, Res Inst Wood Ind, Wood Anat & Utilizat Dept, Beijing, Peoples R China

年份：2017

卷号：8

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000407696900003)】；

基金：This work was supported by National Natural Science Foundation of China (grant No. 31200508).

语种：英文

外文关键词：Ma bamboo; digital gene expression; ploidy; coexpression network; growth properties; anatomical characteristics

摘要：Elucidating the differences in gene expression profiles of plants with different ploidy levels and how they affect phenotypic traits is vital to allow genetic improvement of plants such as Ma bamboo (Dendrocalamus latiflorus Munro). We previously obtained triploid (2n = 3X = 36), hexaploid (2n = 6X = 72), and dodecaploid (2n = 12X = 144) Ma bamboo plants from embryogenic callus by anther culturing. Phenotypic differences between these plants appeared to be correlated with differences in ploidy. Here, we performed transcriptome profiling and sequencing of anther-regenerated plants and F1 seedlings of different ploidy levels using RNA-Seq technology. Pair-wise comparisons of the four resulting libraries revealed 8,396 differentially expressed genes. These differentially expressed genes were annotated, functionally classified, and partially validated. We found that the chromosome doubling led to substantially up- or down-regulation of genes that were involved in cell growth and differentiation; the polyploidy levels altered the anatomical, physiological and growth characteristics, such as leaf thickness, fusoid cell and stomatal size, shoot number, photosynthesis and respiration rate and so on. Additionally, two candidate genes, EXPB3 and TCP with potenitial regulatory roles in cell division and differentiation, were identified through gene coexpresseion network analysis. These results highlight the significance of potential applications of polyploidy, and provide valuable information for the genetic breeding of bamboo species.