文献类型：期刊文献

英文题名：The Populus trichocarpa PtHSP17.8 involved in heat and salt stress tolerances

作者：Li, Jianbo[1] Zhang, Jin[1,2] Jia, Huixia[1,2] Li, Yu[1] Xu, Xiangdong[1] Wang, Lijuan[1] Lu, Mengzhu[1,2]

通信作者：Zhang, J[1];Lu, MZ[1];Zhang, J[2];Lu, MZ[2]|[a0005047f1c42f7291855]张进;

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat, State Forestry Adm,Res Inst Forestry, Beijing 100091, Peoples R China;[2]Nanjing Forestry Univ, Collaborat Innovat Ctr Sustainable Forestry South, Nanjing 210037, Jiangsu, Peoples R China

年份：2016

卷号：35

期号：8

起止页码：1587-1599

外文期刊名：PLANT CELL REPORTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000379851100003)】；

基金：This work was supported by the China Postdoctoral Science Foundation [2014 M550104] to J.Z. and the National Key Basic Research Program of China [2012CB114500] and a Collaborative Innovation Plan of Jiangsu Higher Education to M.L.

语种：英文

外文关键词：PtHSP17.8; Populus; Arabidopsis; Heat; Salt; Tolerance

摘要：PtHSP17.8 was regulated by various abiotic stresses. Overexpression of PtHSP17.8 enhanced the tolerance to heat and salt stresses through maintain ROS homeostasis and cooperate with stress-related genes in Arabidopsis. Small heat shock proteins (sHSPs) play important roles in response to diverse biotic and abiotic stresses, especially in heat tolerance. However, limited information is available on the stress tolerance roles of sHSPs in woody species. To explore the function of sHSPs in poplar, we isolated and characterized PtHSP17.8 from Populus trichocarpa. Phylogenetic analysis and subcellular localization revealed that PtHSP17.8 was a cytosolic class I sHSP. The gene expression profile of PtHSP17.8 in various tissues showed that it was significantly accumulated in stem and root, which was consistent with the GUS expression pattern driven by promoter of PtHSP17.8. The expression of PtHSP17.8 could be induced by various abiotic stresses and significantly activated by heat stress. Overexpression of PtHSP17.8 enhanced the tolerance to heat and salt stresses in Arabidopsis. The seedling survival rate, root length, relative water content, antioxidative enzyme activities, proline, and soluble sugar content were increased in transgenic Arabidopsis under heat and salt stresses, but not in normal condition. The co-expression network of PtHSP17.8 were constructed and demonstrated many stress responsive genes included. The stress-related genes in the co-expression network were up-regulated in the PtHSP17.8 overexpression seedlings. These results suggest that PtHSP17.8 confers heat and salt tolerances in plants.