文献类型：期刊文献

英文题名：Mitochondrial Genome Assembly and Comparative Analysis of Chionanthus Retusus (Oleaceae)

作者：Zhai, Shasha[1] Lin, Furong[2] Shu, Xiuge[3] Niu, Hongyun[4] Jing, Qi[5] Gao, Lei[1] Gao, Xiangbin[1] Liu, Dan[5]

第一作者：Zhai, Shasha

通信作者：Gao, XB[1];Liu, D[2]

机构：[1]Liaocheng Univ, Coll Agr & Biol, Liaocheng 252000, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat State Forestry, Beijing 100091, Peoples R China;[3]Shandong Acad Forestry Sci, Jinan 250014, Peoples R China;[4]Shandong Aviat Emergency Rescue Ctr, Jinan 250014, Peoples R China;[5]Shandong Prov Ctr Forest & Grass Germplasm Resourc, Jinan 250102, Peoples R China

年份：2024

卷号：15

期号：12

外文期刊名：GENES

收录：;Scopus(收录号：2-s2.0-85213215656);WOS:【SCI-EXPANDED(收录号:WOS:001385489000001)】；

基金：This study was funded by the National Forestry and Grassland Germplasm Resources Platform (2005DKA21003), Demonstration and Promotion of Ancient and Famous Trees Protection Technology (Lu [2024] TG08), and Key Research Project of Shandong Province: Research on Key Technologies for Dynamic Monitoring and Regression of Rare and Endangered Germplasm Resources (2021LZGC0230203).

语种：英文

外文关键词：chionanthus retusus; mitochondrial genome; phylogenetic analysis; migration sequence

摘要：Background/Objectives: Chionanthus retusus Lindl. & Paxton is an ornamental tree species native to North China. Research on the mitochondrial genome can elucidate the evolution and biological characteristics of C. retusus and better protect this important species. Methods and Results: This work aimed to clarify the evolutionary and phylogenetic links by sequencing, assembling, annotating, and analyzing the entire mitochondrial genome of C. retusus. The single-loop structure that made up the mitochondrial genome had a total length of 657,640 bp and a GC content of 44.52%. In total, 37 unique protein-coding genes, 20 tRNA genes, and 3 rRNA genes were identified. Numerous repeat sequences and migrating fragments of chloroplast sequences were found. Using the mitochondrial protein-coding genes to construct evolutionary trees, it was found that the closest relative of C. retusus is C. rupicola (Lingelsh.) Kiew. Conclusions: This research represents the first comprehensive set of data on the mitochondrial genome of an ancient (>500 yr) C. retusus specimen. In addition to elucidating the biological characteristics of C. retusus. The findings contribute to the Oleaceae mitochondrial genome database and offer valuable insights for future studies in molecular breeding, evolutionary biology, and genetic diversity conservation.