文献类型：期刊文献

英文题名：Populus euphratica JRL Mediates ABA Response, Ionic and ROS Homeostasis in Arabidopsis under Salt Stress

作者：Zhang, Huilong[1] Deng, Chen[1] Yao, Jun[1] Zhang, Yan-Li[1] Zhang, Yi-Nan[1] Deng, Shurong[1,2] Zhao, Nan[1] Sa, Gang[1] Zhou, Xiaoyang[1] Lu, Cunfu[1] Lin, Shanzhi[1] Zhao, Rui[1] Chen, Shaoliang[1]

第一作者：Zhang, Huilong

通信作者：Zhao, R[1];Chen, SL[1]

机构：[1]Beijing Forestry Univ, Coll Biol Sci & Technol, Beijing Adv Innovat Ctr Tree Breeding Mol Design, Beijing 100083, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China

年份：2019

卷号：20

期号：4

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;Scopus(收录号：2-s2.0-85061610743);WOS:【SCI-EXPANDED(收录号:WOS:000460805400023)】；

基金：This research was supported jointly by National Natural Science Foundation of China (grant nos. 31770643 and 31570587), Beijing Municipal Natural Science Foundation (grant nos. 6182030 and 6172024), the Program of Introducing Talents of Discipline to Universities (111 Project, grant no. B13007), and Fundamental Research Funds for the Central Universities (2017ZY07).

语种：英文

外文关键词：jacalin-related lectin; Populus euphratica; NaCl; antioxidant enzyme; abscisic acid; K+; Na+ homeostasis; non-invasive micro-test technique

摘要：Sodium chloride (NaCl) induced expression of a jacalin-related mannose-binding lectin (JRL) gene in leaves, roots, and callus cultures of Populus euphratica (salt-resistant poplar). To explore the mechanism of the PeJRL in salinity tolerance, the full length of PeJRL was cloned from P. euphratica and was transformed into Arabidopsis. PeJRL was localized to the cytoplasm in mesophyll cells. Overexpression of PeJRL in Arabidopsis significantly improved the salt tolerance of transgenic plants, in terms of seed germination, root growth, and electrolyte leakage during seedling establishment. Under NaCl stress, transgenic plants retained K+ and limited the accumulation of Na+. PeJRL-transgenic lines increased Na+ extrusion, which was associated with the upward regulation of SOS1, AHA1, and AHA2 genes encoding plasma membrane Na+/proton (H+) antiporter and H+-pumps. The activated H+-ATPases in PeJRL-overexpressed plants restricted the channel-mediated loss of K+ that was activated by NaCl-induced depolarization. Under salt stress, PeJRL-transgenic Arabidopsis maintained reactive oxygen species (ROS) homeostasis by activating the antioxidant enzymes and reducing the production of O-2(-) through downregulation of NADPH oxidases. Of note, the PeJRL-transgenic Arabidopsis repressed abscisic acid (ABA) biosynthesis, thus reducing the ABA-elicited ROS production and the oxidative damage during the period of salt stress. A schematic model was proposed to show the mediation of PeJRL on ABA response, and ionic and ROS homeostasis under NaCl stress.