文献类型：期刊文献

中文题名：高含油小球藻(Chlorella sp.)培养工艺条件的研究

英文题名：Investigation of Process to Cultivate High-lipid-content Chlorella sp.

作者：杨静[1] 蒋剑春[1] 张宁[1]

第一作者：杨静

机构：[1]中国林业科学研究院林产化学工业研究所生物质化学利用国家工程实验室国家林业局林产化学工程重点开放性实验室江苏省生物质能源与材料重点实验室

年份：2011

卷号：31

期号：3

起止页码：11-16

中文期刊名：林产化学与工业

外文期刊名：Chemistry and Industry of Forest Products

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD_E2011_2012】；

基金：中国博士后科学基金资助项目(20090460037)

语种：中文

中文关键词：小球藻;油脂产率;部分析因试验;正交试验

外文关键词：Chlorella sp.; lipid productivity; fractional factorial design; orthogonal experiment

分类号：TQ351

摘要：考察了小球藻的异养能力,并利用部分析因试验和正交试验两步试验设计优化了小球藻产油条件。实验结果表明:所选小球藻能够进行异养,在葡萄糖、半乳糖和麦芽糖中均生长良好。以葡萄糖为外加碳源时,兼养组和异养组的生长量较之自养组分别提高了3.72和2.74倍。经过部分析因试验和正交试验,筛选出影响小球藻油脂产率的显著因素为氮源浓度和温度。在氮源浓度为6.0 mmol/L,温度为25℃,pH值为6.0时,小球藻的油脂产率可达到58.49 mg/(L.d)。
The heterotrophic capability of Chlorella sp.was investigated.The conditions of lipid productivity were optimized by fractional factorial design（FFD） and orthogonal experiments.The results showed that Chlorella sp.had the heterotrophic capability and grew well in glucose,galactose and maltose.Compared to that in autotrophy the biomass which grew in mixtrophy and heterotrophy increased by 3.72 times and 2.74 times respectively,using glucose as additional carbon resource.Through FFD and orthogonal experiments,nitrogen source concentration and temperature were selected as significant factors.The highest lipid productivity of 58.49mg/（L·d） occurred under the conditions of nitrogen source concentration 6.0mmol/L,temperature 25℃ and pH value 6.0.GC-MS analysis demonstrated that the fatty acid composition of the lipid was similar to that of vegetable oil and its unsaturated fatty acid content reached 72.97%.It is a promising material for biodiesel production.