文献类型：期刊文献

英文题名：An essential role for Arabidopsis Trs33 in cell growth and organization in plant apical meristems

作者：Zhang, Jin[1,2] Chen, Jun[1,3] Wang, Lijuan[1] Zhao, Shutang[1] Wang, Weina[3] Li, Jianbo[1] Liu, Bobin[1] Qi, Xingyun[3] Zheng, Huanquan[3] Lu, Mengzhu[1,4]

第一作者：Zhang, Jin

通信作者：Lu, MZ[1];Zheng, HQ[2];Lu, MZ[3]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat,State Forestry A, Beijing 100091, Peoples R China;[2]Oak Ridge Natl Lab, Biosci Div, POB 2009, Oak Ridge, TN 37831 USA;[3]McGill Univ, Dept Biol, 1205 Dr Penfield Ave, Montreal, PQ H3A 1B1, Canada;[4]Zhejiang A&F Univ, Sch Forestry & Biotechnol, State Key Lab Subtrop Silviculture, Hangzhou 311300, Zhejiang, Peoples R China

年份：2020

卷号：39

期号：3

起止页码：381-391

外文期刊名：PLANT CELL REPORTS

收录：;Scopus(收录号：2-s2.0-85076337020);WOS:【SCI-EXPANDED(收录号:WOS:000513216200007)】；

基金：This work was supported by the Special Fund on Essential Research for National Non-profit Institutions to the Chinese Academy of Forestry (CAFYBB2011001) to ML, and a Grant from The National Science and Engineering Research Council of Canada and a startup grant from McGill University (Montreal, Canada) to HZ.

语种：英文

外文关键词：Arabidopsis; Trs33; TRAPP; Apical meristem; Auxin; PIN1; 2; Subcellular localization

摘要：Key message Trafficking protein particle (TRAPP) complexes subunit gene AtTrs33 plays an important role in keeping apical meristematic activity and dominance in Arabidopsis. TRAPP complexes, composed of multimeric subunits, are guanine-nucleotide exchange factors for certain Rab GTPases and are believed to be involved in the regulation of membrane trafficking, but the cases in Arabidopsis are largely unknown. Trs33, recently proposed to be a component of TRAPP IV, is non-essential in yeast cells. A single copy of Trs33 gene, AtTrs33, was identified in Arabidopsis. GUS activity assay indicated that AtTrs33 was ubiquitously expressed. Based on a T-DNA insertion line, we found that loss-of-function of AtTrs33 is lethal for apical growth. Knock-down or knock-in of AtTrs33 affects apical meristematic growth and fertility, which indicates that AtTrs33 plays an important role in keeping apical meristematic activity and dominance in Arabidopsis. Analysis of auxin responses and PIN1/2 localization indicate that impaired apical meristematic activity and dominance were caused by altered auxin responses through non-polarized PIN1 localization. The present study reported that AtTrs33 plays an essential role in Arabidopsis cell growth and organization, which is different with its homologue in yeast. These findings provide new insights into the functional divergence of TRAPP subunits.