文献类型：期刊文献

英文题名：Transcriptome and physiological analyses provide insights into the leaf epicuticular wax accumulation mechanism in yellowhorn

作者：Zhao, Yang[1,2,3] Liu, Xiaojuan[1] Wang, Mengke[1] Bi, Quanxin[1] Cui, Yifan[1] Wang, Libing[1]

第一作者：Zhao, Yang

通信作者：Wang, LB[1];Wang, LB[2]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing 100101, Peoples R China;[3]Univ Chinese Acad Sci, Beijing 100039, Peoples R China

年份：2021

卷号：8

期号：1

外文期刊名：HORTICULTURE RESEARCH

收录：;Scopus(收录号：2-s2.0-85107332457);WOS:【SCI-EXPANDED(收录号:WOS:000693639900019)】；

基金：This work was financially supported by the Central Public-Interest Scientific Institution Basal Research Fund (CAFYBB2020ZY003), the Natural Science Foundation of China (31870594) and the "Youth Top Talent Project" of the "Ten Thousand Talents Program" of the State. The funding body was not involved in the design of the study, the collection, analysis, and interpretation of data or in writing the manuscript.

语种：英文

摘要：Plantations and production of yellowhorn, one of the most important woody oil and urban greening trees widely cultivated in northern China, have gradually become limited by drought stress. The epicuticular wax layer plays a key role in the protection of yellowhorn trees from drought and other stresses. However, there is no research on the mechanism of wax loading in yellowhorn trees. In this study, we investigated the anatomical and physiological characteristics of leaves from different germplasm resources and different parts of the same tree and compared their cuticle properties. In addition, the different expression patterns of genes involved in wax accumulation were analyzed, and a coexpression network was built based on transcriptome sequencing data. Morphological and physiological comparisons found that the sun leaves from the outer part of the crown had thicker epicuticular wax, which altered the permeability and improved the drought resistance of leaves, than did shade leaves. Based on transcriptome data, a total of 3008 and 1324 differentially expressed genes (DEGs) were identified between the sun leaves and shade leaves in glossy- and non-glossy-type germplasm resources, respectively. We identified 138 DEGs involved in wax biosynthesis and transport, including structural genes (such as LACS8, ECH1, and ns-LTP) and transcription factors (such as MYB, WRKY, and bHLH transcription factor family proteins). The coexpression network showed a strong correlation between these DEGs. The differences in gene expression patterns between G- and NG-type germplasm resources under different light conditions were very clear. These results not only provide a theoretical basis for screening and developing drought-resistant yellowhorn germplasm resources but also provide a data platform to reveal the wax accumulation process of yellowhorn leaves.