文献类型：期刊文献

英文题名：Transgene expression in Chinese sweetgum driven by the salt induced expressed promoter

作者：Renying, Zhuo[1] Guirong, Qiao[1] Zongxiu, Sun[2]

第一作者：卓仁英

通信作者：Zhuo, RY[1]|[a00058eb5603bedf0c2e1]卓仁英;

机构：[1]Chinese Acad Forest, Res Inst Subtrop Forest, Key Lab Tree Genom, Fuyang 311400, Hangzhou Zhejia, Peoples R China;[2]China Natl Rice Res Inst, State Key Lab Rice Biol, Hangzhou 310006, Zhejiang, Peoples R China

年份：2007

卷号：88

期号：1

起止页码：101-107

外文期刊名：PLANT CELL TISSUE AND ORGAN CULTURE

收录：;EI(收录号：20070410387002);Scopus(收录号：2-s2.0-33846307816);WOS:【SCI-EXPANDED(收录号:WOS:000243559100011)】；

基金：Acknowledgements This work was supported by Science and Technology Department of Zhejiang Province (NO.2004C32002) and 948 Program (No.2005-4-58) by State Forestry Administration P. R. China. The authors thank Scott Merkle for language revision of the manuscript.

语种：英文

外文关键词：Chinese sweetgum; plantlet regeneration; transgenic plantlet; salt induced promoter

摘要：Conventional breeding of Chinese sweetgum is constrained by its long-reproductive cycle, which includes long-juvenile periods, and by its complex reproductive characteristics, including self-incompatibility and a high degree of heterozygosis. Like other tree species, sweetgum has undergone relatively little domestication; the methodology described here in illustrates the possibility of transforming Liquidambar formosana L. obtained from leafy explants using Agrobacter tumefaciens. PCR and Southern blotting show that foreign gene had integrated to genomic DNA. The results indicated that superoxide dismutase (SOD) and peroxidase (POD) activities increased with the stress time in all treated plants and these activities of the transgenic plant were stably higher than those of the control. RT-PCR showed that BADH expressed strongly induced by NaCl. The present study showed that the Rd29A promoter is able to direct osmotant gene expression when plant was exposed to salt, cold, and drought stress, with the advantage that expression was absent or undetectable in natural grow phase.