文献类型：期刊文献

英文题名：Identification of Commercial Cultivars in the Tabebuia Alliance Using Genotyping-by-Sequencing

作者：Meng, Jngxiang[1] Zhang, Yong[1] Wei, Yongcheng[1] Li, Rongrong[2,3] Li, Zhen[4] Zhong, Chonglu[1]

第一作者：Meng, Jngxiang

通信作者：Zhang, Y[1]

机构：[1]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Peoples R China;[2]Northeast Forestry Univ, Coll Landscape Architecture, Harbin 150040, Peoples R China;[3]Novogene Bioinformat Technol Co Ltd, Beijing 100015, Peoples R China;[4]Shanxi Agr Univ, Grad Sch, Jingzhong 030801, Peoples R China

年份：2023

卷号：14

期号：2

外文期刊名：FORESTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000939260800001)】；

基金：This research was funded by Fundamental Research Funds for the Central Non-profit Research Institution of CAF (CAFYBB2020SY021) and Funding by Science and Technology Projects in Guangzhou (SL2022A04J00896).

语种：英文

外文关键词：Tabebuia alliance; genotyping-by-sequencing; molecular marker; species identification; cultivar variation; fingerprints

摘要：The Tabebuia alliance is widely planted in tropical and subtropical countries. However, the lack of accurate taxonomic information for many commercial cultivars leads to commercial disputes. We sought to clarify the background of commercial cultivars in China and to provide a robust basis for patent protection. We collected 82 Tabebuia cultivars and 12 reference individuals, then developed large-scale single nucleotide polymorphism (SNP) markers using genotyping-by-sequencing. We obtained 73,219 SNPs from the test samples, which represented a substantial increase compared with previous studies of Tabebuia species. Based on these SNPs and a clustering analysis, we detected six species among the samples, as well as an uncertain population which may be a hybrid between Handroanthus impetiginosus (Mart. ex DC) Mattos and H. heptaphyllus (Vell.) Mattos. An analysis of genetic diversity to assess germplasm resources indicated that excessive heterozygosity of these cultivars in China, which may explain the genetic basis of "novel-preferred" selection in ornamental plants or a narrow genetic background. After rigorous selection, we identified numerous high-quality molecular markers for cultivar identification, then constructed a concise fingerprint with 54 SNPs. Our work clarifies the background information of commercial cultivars of Tabebuia in China and provides information for cultivar identification. It will serve as an important technical resource for genetic studies, and it will help to protect patents that involve Tabebuia species.