文献类型：期刊文献

英文题名：Soil Bacterial Community Response to Short-Term Manipulation of the Nitrogen Deposition Form and Dose in a Chinese Fir Plantation in Southern China

作者：Liu, Caixia[1,2,3] Dong, Yuhong[1,2,3] Sun, Qiwu[1,2,3] Jiao, Ruzhen[1,2,3]

第一作者：Liu, Caixia

通信作者：Jiao, RZ[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China;[2]State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[3]Key Lab Tree Breeding & Cultivat State Forestry A, Beijing 100091, Peoples R China

年份：2016

卷号：227

期号：12

外文期刊名：WATER AIR AND SOIL POLLUTION

收录：;EI(收录号：20164803051533);Scopus(收录号：2-s2.0-84995938195);WOS:【SCI-EXPANDED(收录号:WOS:000390681500029)】；

基金：This study was supported in part by [1] National Key Research Program of China (No.2016YFD0600302) and [2] Research Institute of Forestry, the Chinese Academy of Forestry (No.CAFYBB2012026).

语种：英文

外文关键词：Soil bacteria; Nitrogen deposition; Colony counting; Phospholipid fatty acid (PLFA); High-throughput sequencing

摘要：The changes of soil bacterial biomass and community composition were monitored in a simulated nitrogen (N) deposition experiment during 4 years of Cunninghamia lanceolata growth in a plantation site in southern China. The experimental design included two N forms (NH4Cl and KNO3) and five levels of N deposition (0, 20, 40, 60, 80 kg N ha(-1)) for 2 years. Research into the bacterial population was conducted using plate count, phospholipid fatty acid (PLFA) composition, and 16Sr DNA gene-based high-throughput pyrosequencing methods. The results of plate count and PLFA analysis indicated that ammonium (NH4+) addition increased bacterial number and biomass, whereas nitrate (NO3-) addition decreased these values. The high-throughput sequencing showed that N deposition of the two N forms inhibited the growth of bacteria compared with control plots, and the changing trend was related to the NH4+-N/NO3--N ratio of soil. When the N deposition dose exceeded 20 kg N ha(-1), there was a significant effect on cultured bacteria counts and bacterial biomass. When examining the bacterial community, we observed 22 bacterial phyla of which Proteobacteria, Acidobacteria, and Actinobacteria were dominant. Acidobacteria abundance was higher in NH4+ treatments than NO3- treatments. When the rates of NH4+ deposition increased, Acidobacteria abundance decreased; however, it showed a positive correlation in NO3- treatments. The bacterial cluster structures were significantly different between different N addition rates in the NO3--treated plots. This research will provide data support to addressing the negative influences of nitrogen deposition and provide reference for soil management.