文献类型：期刊文献

英文题名：DNA barcoding of vouchered xylarium wood specimens of nine endangered Dalbergia species

作者：Yu, Min[1,2] Jiao, Lichao[1,2] Guo, Juan[1,2] Wiedenhoeft, Alex C.[3,4,5,6] He, Tuo[1,2] Jiang, Xiaomei[1,2] Yin, Yafang[1,2]

通信作者：Yin, YF[1];Yin, YF[2]

机构：[1]Chinese Acad Forestry, Chinese Res Inst Wood Ind, Dept Wood Anat & Utilizat, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Wood Collect WOODPEDIA, Beijing 100091, Peoples R China;[3]US Forest Serv, Ctr Wood Anat Res, USDA, Forest Prod Lab, Madison, WI 53726 USA;[4]Univ Wisconsin, Dept Bot, Madison, WI 53706 USA;[5]Purdue Univ, Dept Forestry & Nat Resources, W Lafayette, IN 47907 USA;[6]Univ Estadual Paulista, Ciencias Biol Bot, Sao Paulo, Brazil

年份：2017

卷号：246

期号：6

起止页码：1165-1176

外文期刊名：PLANTA

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000413630100009)】；

基金：This work was supported by the China Postdoctoral Science Foundation (Grant no. 2016M590152), the National Natural Science Foundation of China (Grant no. 31600451), the Fundamental Research Funds of Chinese Academy of Forestry (Grant no. CAFYBB2017ZE003), the China Scholarship Council (Grant no. 2016-3035), and U.S. State Department Interagency Agreement (19318814Y0010-140001-0001/P00001). We thank Sarah Friedrich, Department of Botany, University of Wisconsin-Madison for her technical assistance with the phylogram figure.

语种：英文

外文关键词：DNA barcode reference database; ITS2; trnH-psbA; CITES; Forensic wood identification; Illegal logging

摘要：ITS2+ trnH - psbA was the best combination of DNA barcode to resolve the Dalbergia wood species studied. We demonstrate the feasibility of building a DNA barcode reference database using xylarium wood specimens. The increase in illegal logging and timber trade of CITES-listed tropical species necessitates the development of unambiguous identification methods at the species level. For these methods to be fully functional and deployable for law enforcement, they must work using wood or wood products. DNA barcoding of wood has been promoted as a promising tool for species identification; however, the main barrier to extensive application of DNA barcoding to wood is the lack of a comprehensive and reliable DNA reference library of barcodes from wood. In this study, xylarium wood specimens of nine Dalbergia species were selected from the Wood Collection of the Chinese Academy of Forestry and DNA was then extracted from them for further PCR amplification of eight potential DNA barcode sequences (ITS2, matK, trnL, trnH-psbA, trnV-trnM1, trnV-trnM2, trnC-petN, and trnS-trnG). The barcodes were tested singly and in combination for species-level discrimination ability by tree-based [neighbor-joining (NJ)] and distance-based (TaxonDNA) methods. We found that the discrimination ability of DNA barcodes in combination was higher than any single DNA marker among the Dalbergia species studied, with the best two-marker combination of ITS2+trnH-psbA analyzed with NJ trees performing the best (100% accuracy). These barcodes are relatively short regions (< 350 bp) and amplification reactions were performed with high success (ae90%) using wood as the source material, a necessary factor to apply DNA barcoding to timber trade. The present results demonstrate the feasibility of using vouchered xylarium specimens to build DNA barcoding reference databases.