文献类型：期刊文献

英文题名：Extracellular ATP activates H₂O₂ signaling to mitigate cadmium toxicity by restricting Cd²⁺ entry and triggering the antioxidant system in Arabidopsis

作者：Deng, Shurong[1] Wang, Yang[1] Huang, Chunran[1] Jian, Wei[2] Zhou, Haichao[3] Hussain, Muzammil[4] Pan, Min[5] Ye, Cheng[6] Zhu, Zhengjie[4] Lang, Tao[3,4]

第一作者：邓澍荣

通信作者：Lang, T[1];Zhu, ZJ[2];Lang, T[2]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Silviculture Natl Forestry & Grassland Adm, Beijing 100091, Peoples R China;[2]Peking Univ, Inst Ecol, Coll Urban & Environm Sci, Beijing 100871, Peoples R China;[3]Shenzhen Univ, Coll Life Sci & Oceanog, Shenzhen 518060, Peoples R China;[4]Baise Univ, Coll Agr & Food Engn, Baise 533000, Peoples R China;[5]Hong Kong Metropolitan Univ, Sch Sci & Technol, Hong Kong 999077, Peoples R China;[6]Quanzhou Tongqing Mangrove Technol Co Ltd, Quanzhou 362000, Peoples R China

年份：2025

卷号：352

外文期刊名：PLANT SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001432513600001)】；

基金：This research was financially supported by the National Natural Science Foundation of China (32371826; 32101367) , the Foundation for the Scientific Research Base and Distinguished Talents in Guangxi (Guike AD22035015; Guike AC22080006) , the Guangxi First-class Dis-ciplines (Agricultural Resources and Environment, BSUFCD-KF202402) , the Guangdong Basic and Applied Basic Research Foundation (2022A1515010698) , the Shenzhen Science and Technology Program (JCYJ20220818095601003; KCXFZ20230731092801002) , and the Guangxi Youth Talent Support Program (Zhengjie Zhu) .

语种：英文

外文关键词：Extracellular ATP; H2O2; Cd2+ influx; Antioxidant system; Cd stress; Arabidopsis

摘要：Extracellular ATP (eATP) has recently been considered important in signaling against abiotic stress in plants. However, the potential advantageous mechanisms of eATP in a plant's adaptation to cadmium (Cd) stress are largely unknown. In the present study, using eATP-insensitive mutants, does not respond to nucleotides 1-3/4, we investigated the possible roles and regulatory effects of eATP in mitigating Cd2+ toxicity in Arabidopsis thaliana. The results show that dorn1-3 and dorn1-4 possessed lower germination and root length, but exhibited higher relative electrolyte leakage than those in wildtype (WT) under Cd stress. In addition, CdCl2 caused a marked trend of first increase and then decrease in eATP within the three strains during 24 h of treatment. The Cd2+induced Cd2+ influx in the roots of dorn1-3 and dorn1-4 was notably higher than that in WT, whether in steady or in transient states. Additionally, the application of exogenous ATP-Na2 (an eATP donor) reduced but exogenous PPADS (a specific inhibitor of P2K1) increased the Cd2+-elicited Cd2+ influx. The fluorescence intensities of Cd2+ and H2O2 in the mutants were also notably higher than those in WT. Furthermore, H2O2 signaling could be activated via eATP signaling and inhibit Cd2+ intry under Cd conditions. Under Cd stress, eATP-triggered H2O2 signaling seemed to activate the downstream transcription of genes involved in the antioxidant system, such as AtGR1, AtCAT1, AtGPX8, and AtSOD1/2, and downregulate the relative levels of AtIRT1 and AtIRT2 transcripts. To sum up, through binding to its receptor, P2K1, the Cd-elicited eATP potentially activated the downstream signal H2O2, which could further inhibit Cd entry by downregulating the expression of AtIRTs and remove excess ROS via upregulating genes involved in the antioxidant system, eventually leading to the mitigation of Cd toxicity.