文献类型：期刊文献

英文题名：Denitrifying bacterial communities in surface-flow constructed wetlands during different seasons: characteristics and relationships with environment factors

作者：Wei, Jia-ming[1,2] Cui, Li-juan[3,4,5] Li, Wei[3,4,5] Ping, Yun-mei[3,4,5] Li, Wan[1,2]

第一作者：Wei, Jia-ming

通信作者：Cui, LJ[1];Cui, LJ[2];Cui, LJ[3]

机构：[1]Beijing Construct Engn Grp Environm Remediat Co L, Beijing 100051, Peoples R China;[2]Natl Engn Lab Site Remediat Technol, Beijing 100872, Peoples R China;[3]Chinese Acad Forestry, Inst Wetland Res, Beijing 100091, Peoples R China;[4]Beijing Key Lab Wetland Ecol Funct & Restorat, Beijing 100091, Peoples R China;[5]Beijing Hanshiqiao Natl Wetland Ecosyst Res Stn, Beijing 101399, Peoples R China

年份：2021

卷号：11

期号：1

外文期刊名：SCIENTIFIC REPORTS

收录：;Scopus(收录号：2-s2.0-85101835411);WOS:【SCI-EXPANDED(收录号:WOS:000625352700007)】；

基金：This work was funded by the National Key Research and Development Program of China (2017YFC0506200) and Beijing Hanshiqiao National Wetland Ecosystem Research Station Operation Project.

语种：英文

摘要：Denitrification is an important part of the nitrogen cycle and the key step to removal of nitrogen in surface-flow wetlands. In this study, we explored space-time analysis with high-throughput sequencing to elucidate the relationships between denitrifying bacteria community structures and environmental factors during different seasons. Our results showed that along the flow direction of different processing units, there were dynamic changes in physical and chemical indicators. The bacterial abundance indexes (ACEs) in May, August, and October were 686.8, 686.8, and 996.2, respectively, whereas the Shannon-Weiner indexes were 3.718, 4.303, and 4.432, respectively. Along the flow direction, the denitrifying bacterial abundance initially increased and then decreased subsequently during the same months, although diversity tended to increase. The abundance showed similar changes during the different months. Surface flow wetlands mainly contained the following denitrifying bacteria genus: unclassified Bacteria (37.12%), unclassified Proteobacteria (18.16%), Dechloromonas (16.21%), unranked environmental samples (12.51%), unclassified Betaproteobacteria (9.73%), unclassified Rhodocyclaceae (2.14%), and Rhodanobacter (1.51%). During different seasons, the same unit showed alternating changes, and during the same season, bacterial community structures were influenced by the second genus proportion in different processing units. ACEs were strongly correlated with temperature, dissolved oxygen, and pH. Bacterial diversity was strongly correlated with temperature, electrical conductivity, pH, and oxidation reduction potential. Denitrifying bacteria are greatly affected by environmental factors such as temperature and pH.