文献类型：期刊文献

英文题名：Identification of the phosphate transporter 1 family genes in the Eucalyptus grandis genome and their expression under different phosphate regimes

作者：Li, Juan[1] Xu, Yanli[1] Liu, Jiaru[1] Liu, Jiayue[1] Lu, Zhaohua[2] Xu, Jianmin[2] Li, Guangyou[2]

第一作者：Li, Juan

通信作者：Li, GY[1]

机构：[1]Shanxi Normal Univ, Coll Life Sci, Taiyuan 030031, Peoples R China;[2]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Peoples R China

年份：2026

卷号：27

期号：1

外文期刊名：BMC GENOMICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001681824100003)】；

基金：This research has been supported by the National Key Research and Development Program of China during the 14th five-year plan Period (2022YFD2200203), the Fundamental Research Funds for the Central Non-profit Research Institution of CAF (CAFYBB2022SY017), the Guangzhou Science and Technology plan project (2023A04J0711) and the Young Scientists Fund of the National Natural Science Foundation of China (32201524).

语种：英文

外文关键词：Phosphate transporter 1; Eucalyptus grandis; Bioinformatics; Phosphate starvation

摘要：Background Phosphorus (P) is an essential nutrient for plant growth and development. Phosphate transporter 1 (PHT1) is a transmembrane protein that mediates the uptake and translocation of inorganic phosphate (Pi) in plants. Despite extensive research on the PHT1 family across various species, the PHT1 genes in Eucalyptus grandis are poorly documented and not identified comprehensively. Results In this study, a total of 21 EgrPHT1genes (EgrPHT1-1 similar to EgrPHT1-21) were identified for the comprehensive analysis of the whole genome of E. grandis. Gene structure showed that the number of exons ranges from 1 to 6, and 57% gene members lacking introns. Protein multi-sequence alignment analysis revealed that all members except the EgrPHT1-5 had 12 transmembrane domains. Chromosome localization analysis showed that all EgrPHT1 genes were unevenly distributed on 8 chromosomes. Collinearity result identified one pair of tandem duplication and two pairs of fragment duplications. Promoter cis-elements suggested that the expression of the EgrPHT1 genes was affected by various hormonal and abiotic stresses. Tissue-specific expression patterns indicated six genes (EgrPHT1-9/10/13/15/16/17) is specifically expressed in the roots, indicating that they may be involved in the formation and development of the root. Furthermore, 9 EgrPHT1 gene members (EgrPHT1-1/3/4/10/11/12/15/16/19) were upregulated in the root and EgrPHT1-1/3/4/1/16/19 was also upregulated in leaves under phosphorus deficiency. Conclusions In this study, we identified and analyzed EgrPHT1 family genes using bioinformatics approaches, and also examined differential expression patterns of EgrPHT1 genes in various tissues and in response to Pi starvation stress, suggesting their involvement in P uptake, translocation, and homeostasis. This information provides a platform for further investigation of their biological functions.