文献类型：期刊文献

中文题名：红树林湿地烷烃降解菌的分离筛选

英文题名：Isolation and screening of hydrocarbon-degrading bacterial strains from mangrove wetland soils

作者：李玫[1] 廖宝文[1]

第一作者：李玫

机构：[1]中国林业科学研究院热带林业研究所

年份：2013

期号：1

起止页码：40-43

中文期刊名：生态科学

外文期刊名：Ecological Science

收录：北大核心:【北大核心2011】；CSCD:【CSCD_E2013_2014】；

基金：中央级公益性科研院所基本科研业务费专项资金(RITFYWZX201111)

语种：中文

中文关键词：红树林湿地;烷烃降解菌;分离筛选

外文关键词：mangrove wetland;hydrocarbon-degrading bacteria;isolation and screening

分类号：S796;S714

摘要：从受石油污染的红树林湿地土样中分离筛选对烷烃具较高降解能力的细菌菌株,以期应用于被石油污染滨海湿地的生物修复。采用富集培养方法,富集、分离和筛选烷烃降解菌;观察各菌落及菌体形态特征;测试菌株Z2的生理生化特征,并用16S rDNA序列分析方法进行该菌种鉴定。分离筛选得到Z1、Z2和Z3这3个能以正十六烷为唯一碳源生长的菌株,其降解率依次为63.4%、82.5%和78.3%,其中菌株Z2的降解率最高。经过形态学观察、生理生化特性实验和16S rDNA序列分析鉴定,菌株Z2为不动杆菌(Acinetobacter sp.)。
Some hydrocarbon-degrading bacterial strains were isolated from the oil-contaminated mangrove soil on Qi-ao Island of Zhuhai City through selective enriched culture.There were three strains（named Z1,Z2 and Z3） being able to grow with hexadecane as sole carbon and energy resources.After 5 days culture,the degradation rates of Z1,Z2 and Z3 were 63.4%,82.5% and 78.3%,respectively.The morphological and physiological-biochemical identification,16S rDNA sequence analysis,and phylogenetic study showed that the strain Z2 belonged to genus Acinetobacter.