文献类型：期刊文献

英文题名：Molecular insights into lignin biosynthesis on cadmium tolerance: Morphology, transcriptome and proteome profiling in Salix matsudana

作者：Yu, Miao[1,2] Zhuo, Renying[1] Lu, Zhuchou[1] Li, Shaocui[1] Chen, Juanjuan[1] Wang, Yujun[1] Li, Jihong[3] Han, Xiaojiao[1]

第一作者：Yu, Miao

机构：[1] State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding of Zhejiang Province, Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Zhejiang, Hangzhou, 311400, China; [2] Forestry Faculty, Nanjing Forestry University, Jiangsu, Nanjing, 210037, China; [3] Forestry College of Shandong Agricultural University, Shandong, Taian, 271018, China

年份：2023

卷号：441

外文期刊名：Journal of Hazardous Materials

收录：EI(收录号：20223712728154);Scopus(收录号：2-s2.0-85137628648)

语种：英文

外文关键词：Biochemistry - Bioremediation - Biosynthesis - Genes - Morphology - Plants (botany) - Proteins - Soil pollution - Value engineering

摘要：Soil pollution caused by cadmium (Cd) is a serious concern. Phytoremediation is a popular technology in the remediation of Cd-contaminated soil. Salix matsudana var. matsudana f. umbraculifera Rehd. has been characterized as a high Cd-accumulating and tolerant willow (HCW). Here, transcriptome and proteome profiling, along with morphology analyses were performed to explore molecular cross-talk involved in Cd tolerance. Our results showed that 73%? 83% of the Cd in roots accumulated in the cell walls and root xylem cell walls were significantly thickened. From transcriptome and proteome analysis, a total of 153 up-regulated differentially-expressed genes and 655 up-regulated differentially-expressed proteins were found in common between two comparison groups (1 d and 4 d vs. respective control). Furthermore, phenylpropanoid biosynthesis was identified as a key pathway in response to Cd stress. In this pathway, lignin biosynthesis genes or proteins were significantly up-regulated, and lignin content increased significantly in roots under Cd stress. Two Cd-induced genes cinnamoyl-CoA reductase 1 (SmCCR1) and cinnamyl alcohol dehydrogenase 7 (SmCAD7) from HCW increased the lignin content and enhanced Cd tolerance in transgenic poplar calli. These results lay the foundation for further clarifying the molecular mechanisms of Cd tolerance in woody plants. ? 2022 Elsevier B.V.