文献类型：期刊文献

英文题名：Characterization of the Transcriptome and Gene Expression of Tetraploid Black Locust Cuttings in Response to Etiolation

作者：Lu, Nan[1,2] Dai, Li[1] Luo, Zijing[1] Wang, Shaoming[3] Wen, Yanzhong[4] Duan, Hongjing[1] Hou, Rongxuan[1] Sun, Yuhan[1] Li, Yun[1]

第一作者：卢楠;Lu, Nan

通信作者：Li, Y[1]

机构：[1]Beijing Forestry Univ, Beijing Adv Innovat Ctr Tree Breeding Mol Design, Key Lab Genet & Breeding Forest Trees & Ornamenta, Natl Engn Lab Tree Breeding,Minist Educ,Coll Biol, Beijing 100083, Peoples R China;[2]Chinese Acad Forestry, State Key Lab Forest Genet & Tree Breeding, Key Lab Tree Breeding & Cultivat State Forestry A, Res Inst Forestry, Beijing 100091, Peoples R China;[3]State Owned Luoning Cty Lv Cun Forest Farm, Luoning 471711, Peoples R China;[4]State Owned Ji Cty Nursery Forests, Linfen 042200, Peoples R China

年份：2017

卷号：8

期号：12

外文期刊名：GENES

收录：;Scopus(收录号：2-s2.0-85036594194);WOS:【SCI-EXPANDED(收录号:WOS:000419212400006)】；

基金：The authors appreciate the financial support from the Fundamental Research Funds for the Central Universities (2016BLPX13), the Scientific Research of the Forest Public Welfare Industry (201304116), and the National Science Foundation of China (31570677).

语种：英文

外文关键词：Tetraploid Robinia pseudoacacia L; etiolation; transcriptome; phytohormone; peroxidase activity

摘要：Etiolation (a process of growing plants in partial or complete absence of light) promotes adventitious root formation in tetraploid black locust (Robinia pseudoacacia L.) cuttings. We investigated themechanismunderlying howetiolation treatment promotes adventitious root formation in tetraploid black locust and assessed global transcriptional changes after etiolation treatment. Solexa paired-end sequencing of complementary DNAs (cDNAs) from control (non-etiolated, NE) and etiolated (E) samples resulted in 107,564 unigenes. In total, 52,590 transcripts were annotated and 474 transcripts (211 upregulated and 263 downregulated) potentially involved in etiolation were differentially regulated. These genes were associated with hormone metabolism and response, photosynthesis, signaling pathways, and starch and sucrose metabolism. In addition, we also found significant differences of phytohormone contents, activity of following enzymes i.e., peroxidase, polyphenol oxidase and indole acetic acid oxidase between NE and E tissues during some cottage periods. The genes responsive to etiolation stimulus identified in this study will provide the base for further understanding how etiolation triggers adventitious roots formation in tetraploid black locus.