文献类型：期刊文献

中文题名：复合诱变选育衣康酸高产菌株的研究

英文题名：Compound Mutation Breeding of Strain with High Itaconic Acid Yield

作者：杨静[1,2,3,4] 蒋剑春[1,2,3,4] 张飞[5] 张宁[1,2,3,4] 卫民[1,2,3,4] 赵剑[1,2,3,4]

第一作者：杨静

机构：[1]中国林业科学研究院林产化学工业研究所;[2]生物质化学利用国家工程实验室;[3]国家林业局林产化学工程重点开放性实验室;[4]江苏省生物质能源与材料重点实验室;[5]江苏警官学院刑事科学技术系

年份：2015

卷号：49

期号：3

起止页码：13-16

中文期刊名：生物质化学工程

外文期刊名：Biomass Chemical Engineering

收录：北大核心:【北大核心2014】；

基金：中国林科院中央级公益性科研院所基本科研业务费专项资金(CAFYBB2014QA023);"十二五"国家科技支撑计划资助(2014BAD02B02)

语种：中文

中文关键词：土曲霉;衣康酸;复合诱变

外文关键词：Aspergillus terreus ; itaconicacid; compound mutation

分类号：TQ35

摘要：采用紫外线-Li Cl、硫酸二乙酯(DES)复合诱变,经平板菌落筛选、摇瓶筛选以及连续传代稳定性考察,从土曲霉(Aspergillus terreus)2433出发菌株获得一株衣康酸高产突变株土曲霉At DES-11,其产酸量达到48.22 g/L,较出发菌株提高了156.08%;糖酸转化率为61.82%,较出发菌株提高了101.36%,且连续传代5次后,遗传性状仍较稳定,产酸量稳定在48 g/L左右,菌落及菌丝形态基本不变。
By the compound mutation of UV-LiC1 and diethyl sulfate （DES）, fiat screening of colony, shaker screening, and stability test of continuous passage, a mutant strain of Aspergillus terreus At DES-11 with high yield of itaconic acid was obtained from A. terreus2433. Its itaconie acid productivity and conversion rate of glucose were 48.22 g/L and 61.82% with an increase of 156.08% and 101.36% compared with those of original strain ,respectively. And the heredity characteristics of the mutant strain were stable after cuhivation for five continuous generations.