文献类型：期刊文献

中文题名：A cDNA microarray analysis of the molecular control of poplar wood properties

英文题名：A cDNA microarray analysis of the molecular control of poplar wood properties

作者：Qinjun Huang[1,2] Changjun Ding[1,2] Weixi Zhang[1,2] Bingyu Zhang[1,2] Yanguang Chu[1,2] Dahai Wang[1,2] Xiaohua Su[1,2]

第一作者：Qinjun Huang;黄秦军

机构：[1]State Key Laboratory of Tree Genetics and Breeding,Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China;[2]Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration,Beijing 100091,China

年份：2017

卷号：28

期号：1

起止页码：71-82

中文期刊名：林业研究：英文版

收录：CSTPCD;;Scopus;CSCD:【CSCD2017_2018】；

基金：supported by the State Key Basic Research Program of China(No.2012CB114506)

语种：英文

中文关键词：cDNA文库;分子生物学;木材性状;微阵列分析;杨树;控制;基因表达模式;相关基因

外文关键词：Populus deltoides ;cDNA microarray ; Expression profile; Wood density ; Microfibfillar angle

分类号：Q78;Q7

摘要：Molecular biological research into wood development and formation has been the focus in recent years,but the pace of discovery of related genes and their functions in the control of wood properties has been slow. The microarray technique—with its advantages of high throughput capacity, sensitivity, and reliability over other tools developed for investigating genes expression patterns—is capable of rapidly assaying thousands of genes.In this study, a cDNA microarray prepared from two cDNA libraries of developing poplar xylem tissues was used to assay gene expression patterns in immature xylem tissues at different heights from the main stem of Populus deltoides(15 years old), which was confirmed to have distinct wood properties(microfibrillar angle, woody density) by X-ray. Two hundred seventy-four transcripts with differential expression profiles between the chips were screened out, and the individual clones were subjected to 5' sequencing. Using bioinformatic analysis, we identifiedcandidate genes that may influence poplar wood properties,many of which belong to various regulatory and signal transduction gene families, such as zinc finger protein transcription factor, DNA-binding transcription factor,ethylene response factors, and so on. The results suggest that these genes may regulate enzymes involved in wood formation. Further work will be performed to clone these genes and determine how they influence poplar wood properties.
Molecular biological research into wood development and formation has been the focus in recent years, but the pace of discovery of related genes and their functions in the control of wood properties has been slow. The microarray technique--with its advantages of high throughput capacity, sensitivity, and reliability over other tools developed for investigating genes expression patterns-is capable of rapidly assaying thousands of genes. In this study, a cDNA microarray prepared from two cDNA libraries of developing poplar xylem tissues was used to assay gene expression patterns in immature xylem tissues at different heights from the main stem of Populus deltoides （15 years old）, which was confirmed to have distinct wood properties （microfibrillar angle, woody density） by X-ray. Two hundred seventy-four transcripts with differ- ential expression profles between the chips were screened out, and the individual clones were subjected to 5＇ sequencing. Using bioinformatic analysis, we identified candidate genes that may influence poplar wood properties, many of which belong to various regulatory and signal transduction gene families, such as zinc finger protein transcription factor, DNA-binding transcription factor, ethylene response factors, and so on. The results suggest that these genes may regulate enzymes involved in wood formation. Further work will be performed to clone these genes and determine how they influence poplar wood properties.