文献类型：期刊文献

英文题名：Assembly of selenium nanoparticles by protein coronas composed of yeast protease A

作者：Nie, Xinling[1,2] Zhu, Zhenze[3] Lu, Honglin[1,2] Xue, Maoyuan[1,2] Tan, Zhongbiao[1,2] Zhou, Jia[1,2] Xin, Ya[1,2] Mao, Yanyong[1,2] Shi, Hao[1,2] Zhang, Daihui[4]

第一作者：Nie, Xinling

通信作者：Shi, H[1];Zhang, DH[2]

机构：[1]Huaiyin Inst Technol, Sch Life Sci & Food Engn, Huaian 223003, Peoples R China;[2]Huaiyin Inst Technol, Jiangsu Prov Engn Lab Biomass Convers & Proc Integ, Huaian 223003, Peoples R China;[3]Southwest Jiaotong Univ, Sch Mat Sci & Engn, Chengdu 611756, Peoples R China;[4]Chinese Acad Forestry, Inst Chem Ind Forest Prod, Nanjing 210042, Peoples R China

年份：2023

卷号：129

起止页码：140-149

外文期刊名：PROCESS BIOCHEMISTRY

收录：;EI(收录号：20231313805399);Scopus(收录号：2-s2.0-85150834274);WOS:【SCI-EXPANDED(收录号:WOS:001055000300001)】；

基金：This work was fi nancially supported by the Postgraduate Research & Practice Innovation Program of Jiangsu Province, China (SJCX22_1669) and Key R & D Plan Cultivation Project of Huaiyin Institute of Technol- ogy, China (22HGZ001) . We would like to thank Editage ( www.editage. cn) for English language editing.

语种：英文

外文关键词：Nanomaterials; Protease A; Protein corona; Selenium nanoparticle

摘要：The biosafety profile of protein corona-encapsulated selenium nanoparticles (SeNPs) has stimulated great in-terest among biomedical and therapeutic researchers. However, elucidating the potential mechanisms underlying the assembly process remains a significant challenge. Herein, we synthesized protein corona-encapsulated SeNPs using Saccharomyces boulardii, and then systematically investigated the complex constituents. The corona-forming protein constituents were analyzed after separation and purification. Protein sequencing results confirmed that PEP4p vacuolar aspartyl protease (protease A, PrA) participates in the SeNP-encapsulating protein corona assembly process. Additionally, protein-encapsulated SeNPs were produced using wild-type and mutant strains to determine the potential mechanism of corona formation. Phylogenetic analysis showed that the mutant strain promoted the accumulation of SeNPs and facilitated extensive production of protein corona-SeNP complexes, which is a universal cellular stress response among yeasts. This study provides an important theoretical and practical basis for the synthesis of various protein corona-coated NP complexes. The protein corona-encapsulated SeNPs fell within an acceptable size range and were effectively protein coated, indicating their potential for nutritional and pharmaceutical applications for humans and animals.