文献类型：期刊文献

英文题名：Cytological and Transcriptome Analyses Provide Insights into Persimmon Fruit Size Formation (Diospyros kaki Thunb.)

作者：Li, Huawei[1,2] Suo, Yujing[2] Li, Hui[3] Sun, Peng[2] Li, Shuzhan[1] Yuan, Deyi[1] Han, Weijuan[2] Fu, Jianmin[2]

第一作者：Li, Huawei

通信作者：Yuan, DY[1];Fu, JM[2]

机构：[1]Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, 498 Shaoshan South Rd, Changsha 410004, Peoples R China;[2]Chinese Acad Forestry, Res Inst Nontimber Forestry, 3 Weiwu Rd, Zhengzhou 450003, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forestry Policy & Informat, Xiangshan Rd, Beijing 100091, Peoples R China

年份：2024

卷号：25

期号：13

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;Scopus(收录号：2-s2.0-85198471174);WOS:【SCI-EXPANDED(收录号:WOS:001270264800001)】；

基金：This research was funded by the National Key R&D Program of China (2018YFD1000606).

语种：英文

外文关键词：persimmon; fruit size; cell number; cell division; ethylene

摘要：Persimmon (Diospyros kaki Thunb.) fruit size variation is abundant. Studying the size of the persimmon fruit is helpful in improving its economic value. At present, the regulatory mechanism of persimmon fruit size formation is still unclear. In this study, the mechanism of fruit size formation was investigated through morphological, cytological and transcriptomic analyses, as well as exogenous ethrel and aminoethoxyinylglycine (AVG: ethylene inhibitor) experiments using the large fruit and small fruit of 'Yaoxianwuhua'. The results showed that stages 3-4 (June 11-June 25) are the crucial morphological period for differentiation of large fruit and small fruit in persimmon. At this crucial morphological period, the cell number in large fruit was significantly more than that in small fruit, indicating that the difference in cell number is the main reason for the differentiation of persimmon fruit size. The difference in cell number was caused by cell division. CNR1, ANT, LAC17 and EB1C, associated with cell division, may be involved in regulating persimmon fruit size. Exogenous ethrel resulted in a decrease in fruit weight, and AVG treatment had the opposite effect. In addition, LAC17 and ERF114 were upregulated after ethrel treatment. These results indicated that high ethylene levels can reduce persimmon fruit size, possibly by inhibiting cell division. This study provides valuable information for understanding the regulation mechanism of persimmon fruit size and lays a foundation for subsequent breeding and artificial regulation of fruit size.