文献类型：期刊文献

英文题名：CcBLH6, a bell-like homeodomain-containing transcription factor, regulates the fruit lignification pattern

作者：Yan, Chao[1,2,3] Hu, Zhikang[1,2] Nie, Ziyan[1,2,4] Li, Jiyuan[2] Yao, Xiaohua[2] Yin, Hengfu[1,2]

通信作者：Yin, HF[1];Yao, XH[2];Yin, HF[2]|[a00051e90d3009f1c7c75]姚小华;

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, State Key Lab Tree Genet & Breeding, Hangzhou 311400, Zhejiang, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Key Lab Forest Genet & Breeding, Hangzhou 311400, Zhejiang, Peoples R China;[3]Chinese Acad Forestry, Expt Ctr Subtrop Forestry, Fenyi 336600, Jiangxi, Peoples R China;[4]Anhui Agr Univ, Sch Forestry & Landscape Architecture, Hefei 230000, Peoples R China

年份：2021

卷号：253

期号：5

外文期刊名：PLANTA

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000725340700003)】；

基金：We would like to thank Dr. Haiping Yu from China National Rice Research Institute (Hangzhou, China) for technical supports on the confocal microscopy analysis. This work was supported by National Key R&D Program of China (2019YFD1000400 and 2019YFD1001602), Nonprofit Research Projects (CAFYBB2018QA005, CAFYBB2018QA006) of Chinese Academy of Forestry, and National Science Foundation of China (31870578).

语种：英文

外文关键词：BELL-like homeodomain gene; Camellia chekiangoleosa; Fruit development; Lignification; TALE transcription factor

摘要：Main conclusion CcBLH6 is a bell-like homeodomain-containing transcription factor that plays an important role of lignin biosynthesis in the control of fruit lignification pattern in Camellia chekiangoleosa. The fruit of Camellia chekiangoleosa has a unique lignification pattern that features with a thick pericarp containing a low level of lignification. Yet the fruit lignification pattern and the regulatory network of responsible gene transcription are poorly understood. Here, we characterized a bell-like homeodomain-containing (BLH) transcription factor from C. chekiangoleosa, CcBLH6, in the control of fruit lignification. CcBLH6 expression was highly correlated with the unique lignification pattern during fruit development. The ectopic expression of CcBLH6 promoted the lignification process of stem and root in Arabidopsis. We found that expression of genes related to lignin biosynthesis and its transcriptional regulation was altered in transgenic lines. In a Camellia callus-transformation system, overexpression of CcBLH6 greatly enhanced the expression of genes related to lignin biosynthesis and its transcriptional regulation was altered in transgenic lines. In the callus-transformation system, overexpression of CcBLH6 greatly enhanced the lignification of parenchymal cells, and the regulation of several genes involved in lignin accumulation was largely consistent between Arabidopsis and Camellia. Our study reveals a positive role of CcBLH6 in the regulation of lignin biosynthesis during fruit lignification in Camellia.