文献类型：期刊文献

英文题名：Functional Characterization of Abdominal-A in the Pine Caterpillar Moth, Dendrolimus punctatus

作者：Liu, Huihui[1] Chen, Lin[1] Ma, Qinghua[1] Wang, Xinghong[1] Zhang, Sufang[2] Zhou, Xuguo[3]

第一作者：刘慧慧

通信作者：Liu, HH[1];Zhang, SF[2];Zhou, XG[3]

机构：[1]Chinese Acad Forestry, Expt Ctr Forestry North China, Natl Permanent Sci Res Base Warm Temperate Zone Fo, Beijing 102300, Peoples R China;[2]Chinese Acad Forestry, Key Lab Forest Protect, Natl Forestry & Grassland Adm, Ecol & Nat Conservat Inst, Beijing 100091, Peoples R China;[3]Univ Illinois, Coll Liberal Arts & Sci, Sch Integrat Biol, Dept Entomol, Urbana, IL 61801 USA

年份：2024

卷号：15

期号：7

外文期刊名：FORESTS

收录：;EI(收录号：20243116781075);Scopus(收录号：2-s2.0-85199595697);WOS:【SCI-EXPANDED(收录号:WOS:001278651900001)】；

基金：This research was supported by the National Natural Science Foundation of China (32371895) and the Fundamental Research Funds for the Central Non-profit Research Institution of CAF (CAFYBB2020QC001).

语种：英文

外文关键词：CRISPR/Cas9; Dendrolimus punctatus; Abdominal-A; segmentation; posterior segment identity

摘要：Hox genes, specifically the bithorax complex (ubx, abdominal-a, and abdominal-b), play a crucial role in specifying posterior abdominal development and serve as key regulators of germline gene development in insects. However, the function of the bithorax complex in the pine caterpillar moth, Dendrolimus punctatus, a major pine tree defoliator in China, remains largely unknown. Specifically, Abdominal-A (Abd-a) controls regional variation in abdominal segmentation in model insects such as Drosophila and Tribolium; however, its role in D. Punctatus remains unexplored. In this study, CRISPR/Cas9 was used to functionally characterize Abd-a in D. punctatus. Two target sites were selected, and the genotypes and phenotypes of the G0 and G1 generations were evaluated. Our findings indicate that knocking out Abd-a led to an abnormality in the posterior segments A2-A7, as well as the loss of appendages, mainly prolegs, and affected the thoracic T3 segmentation as well as wing development. Moreover, mutation in Abd-a also impacted anal and reproductive development. Taken together, these results demonstrate that DpAbd-a is essential for embryonic and reproductive development in D. punctatus and could be a promising target for genetic control of this devastating conifer defoliator.