文献类型：期刊文献

中文题名：板栗与锥栗杂交F_(1)代叶片表型变异及杂种优势研究

英文题名：Leaf Phenotype Variation and Heterosis in F_(1) Progeny of Cross Between Castanea mollissima and C.henryi

作者：章平生[1,2] 江锡兵[1] 龚榜初[1] 徐阳[1] 赖俊声[3] 吴聪连[3]

第一作者：章平生

机构：[1]中国林业科学研究院亚热带林业研究所,杭州311400;[2]南京林业大学,南京210037;[3]庆元县林业局,庆元323800

年份：2021

卷号：41

期号：2

起止页码：281-293

中文期刊名：植物研究

外文期刊名：Bulletin of Botanical Research

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：中央级公益性科研院所基本科研业务费专项资金重点项目(CAFYBB2017ZA004-6);浙江省农业(林木)新品种选育重大科技专项课题(2016C02056-6)。

语种：中文

中文关键词：栗属;杂交子代;遗传变异;杂种优势

外文关键词：Castanea;hybrid offspring;genetic variation;heterosis

分类号：S792.17

摘要：为探索栗属杂交F_(1)代幼林期叶片性状遗传变异规律及杂种优势,以板栗、锥栗种内和种间9个杂交组合235个杂交单株及其亲本为研究材料,利用巢式方差分析、Pearson相关性分析及主成分分析等统计学方法对其14个叶表型及光合生理性状进行分析。结果表明:①栗属杂交F_(1)代叶表型及光合生理性状存在丰富的遗传多样性,14个性状平均变异系数范围为4.40%~27.76%;②性状组合间表型分化系数VST均值为32.32%,组合内变异是主要的变异来源;③性状中亲优势率为-27.16%~90.53%,且不同组合性状杂种优势存在差异;④Pearson相关性分析结果显示14个性状中分别有46对和13对相关性达到极显著(P<0.01)和显著水平(P<0.05),其中叶长、叶宽等5个叶表型性状及叶绿素a、叶绿素b等4个光合生理指标相互间影响较大;⑤利用主成分分析法筛选出“魁栗×YLZ 15号”和“魁栗×YLZ 1号”两个优良杂交组合,其子代叶片具有叶面积大、干物质含量及光合有关色素含量高的特点,可为进一步筛选优良杂交子代提供主要素材。
In order to explore the genetic variation and heterosis of leaf traits in cross F_(1) progeny of Castanea,14 traits of leaf phenotype and photosynthetic physiology of F_(1) progeny(including 235 individuals)of 9 combinations(including interspecific and intraspecific cross between C.mollissima and C.henryi)and their parents were studied by using the statistical methods of Nested anova,Pearson correlation analysis and principal component analysis.The results showed:①The average coefficient of variation of 14 traits ranged from 4.40%-27.76%,indicating that there were abundant genetic diversity in leaf phenotype and photosynthetic physiological traits in F_(1) hybrid progeny of Castanea.②The average of phenotypic differentiation coefficient among combination were 32.32%,which showed that the variation within combinations was the main source of variation.③The mid-parent heterosis rate of 14 traits ranged from-27.16%-90.53%,and the heterosis of different combinations was different.④The Pearson correlation analysis showed that there were 46 and 13 pairs of correlations reached extremely significant levels(P<0.01)and significant levels(P<0.05),respectively,and 5 leaf phenotype traits,such as leaf length and leaf width,and 4 photosynthetic physiological indexes,such as chlorophyll a and chlorophyll b,had a great influence on each other.⑤2 crossing combinations‘Kuili’×YLZ No.15 and‘Kuili’×YLZ No.1 were finally selected by principal component analysis,and their offspring had the characteristics of large leaf area,high dry matter and photosynthetic pigment content,which can provide the main materials for further screening of excellent offspring.