文献类型：期刊文献

英文题名：Four abiotic stress-induced miRNA families differentially regulated in the embryogenic and non-embryogenic callus tissues of Larix leptolepis

作者：Zhang, Shougong[1] Zhou, Jian[2] Han, Suying[3] Yang, Wenhua[1] Li, Wanfeng[1] Wei, Huali[1] Li, Xinmin[2,3] Qi, Liwang[1]

通信作者：Qi, LW[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Cell Biol Lab, Beijing 100091, Peoples R China;[2]Univ Calif Los Angeles, Dept Pathol & Lab Med, Los Angeles, CA 90095 USA;[3]Chinese Acad Forestry, Key Lab, Res Inst Forest Ecol & Protect, Beijing 100091, Peoples R China

年份：2010

卷号：398

期号：3

起止页码：355-360

外文期刊名：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000280867400004)】；

基金：This work was supported by the National Basic Research Program of China (Grant No. 2009CB119100), the National Natural Science Foundation of China (Grant No. 30830086), National High Technology Research and Development Program of China (Grant Nos. 2006AA100109, 2007AA021403, 2007AA10Z182, 2008AA10Z126 and 2007AA021501), and National Bureau of Forestry "948" Program (Grant No. 2007-4-03).

语种：英文

外文关键词：Larix leptolepis; miRNA regulation; Embryogenesis; miR171; miR172

摘要：Somatic embryogenesis involves complex molecular signaling pathways. Deregulation of these signaling pathways can transform the emblyogenic callus to non-embryogenic callus. To investigate the miRNA regulation underlying this detrimental transformation in Japanese Larch (Larix leptolepis), we compared miRNA expression profiles between embryogenic and non-embryogenic callus at day 3 and day 14 after sub-culture. Four miRNA families dominated the 165 differentially expressed miRNAs between embryogenic and non-embryogenic callus. Of the four, miR171 was up-regulated, and miR159, miR169, and miR172 were down-regulated in the embryogenic callus. These four families are all abiotic stress-induced miRNAs, and all target transcription factors that regulate a group of genes important for cell differentiation and development, including scarecrow-like (SCL) transcription factor (miR171), apetala2 (miR172), MYB transcription factors (miR159), and NF-YA transcription factor (miR169). Three down-regulated miRNA families in the embryogenic callus are also regulated by ABA, which further shed light into the potential mechanisms underlying the transformation of the embryogenic competence in L. leptolepis. This study represents the first report on the miRNA regulation of the embryogenic and non-embryogenic callus in plant, and thus these four miRNA families provide important clues for further functional investigation. (C) 2010 Elsevier Inc. All rights reserved.