文献类型：期刊文献

中文题名：流动分析仪同时快速测定植物全氮、全磷含量的方法改进

英文题名：Improvement of simultaneous and rapid determination of total nitrogen and total phosphorus in plants by flow analyzer

作者：李朝英[1] 郑路[1,2]

第一作者：李朝英

机构：[1]中国林业科学研究院热带林业实验中心,广西凭祥532600;[2]广西友谊关森林生态系统国家定位观测研究站,广西凭祥532600

年份：2021

期号：2

起止页码：336-342

中文期刊名：中国土壤与肥料

外文期刊名：Soil and Fertilizer Sciences in China

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD_E2021_2022】；

基金：南亚热带人工林生态水文过程及机制观测研究(桂科AD20159094);广西友谊关森林生态系统国家定位观测研究站运行补助(2018-LYPT-DW-075)。

语种：中文

中文关键词：流动分析仪;植物;全氮;全磷;方法改进

外文关键词：flow analyzer;plant;total nitrogen;total phosphorus;method improvement

分类号：R28

摘要：为了充分发挥流动分析仪的检测优势,准确高效地同时测定植物全氮、全磷含量,对现有方法提出改进,并将改进方法与传统方法的测定结果进行比较分析,探讨流动分析仪同时测定植物全氮、全磷含量改进方法的可行性。结果表明,经t检验改进方法与传统的扩散法所测全氮含量无显著性差异(P>0.05),改进方法与传统的钼锑抗比色法所测全磷含量无显著性差异(P>0.05)。改进方法与扩散法测定全氮含量回归方程为:Y(扩散法-N)=0.978X(流动分析仪-N)+0.502(r~2=0.9755,P<0.01),两种方法所测全氮呈极显著性相关。改进方法与钼锑抗比色法测定全磷含量回归方程Y(钼锑抗比色法-P)=0.985X(流动分析仪-P)+0.035(r~2=0.9835,P<0.01),两种方法所测全磷呈极显著性相关。流动分析仪测定植物全氮、全磷含量的改进方法加标回收率分别在98.23%~100.68%、98.67%~99.11%之间,3个样品重复测定4次全氮、全磷含量的相对标准偏差均小于6%。流动分析仪同时测定全氮、全磷含量的改进方法操作简捷,精密度与准确性高,试剂用量少,检测效率高,适用于大批量植物的检测分析。
In order to make full use of the advantages of flow analyzer,and accurately and efficiently determine the total nitrogen and total phosphorus content of plants at the same time,the existing method was improved,and the improved method was compared and analyzed with that of the traditional method.The feasibility of improving the method for simultaneous determination of total nitrogen and total phosphorus in plants by flow analyzer was discussed.The results showed that there was no significant difference of total nitrogen content by improved method and the diffusion method(P>0.05),and there was no significant difference of total phosphorus by improved method and the molybdenum-antimony colorimetric method(P>0.05).The regression equation of improved method and diffusion method to determine the total nitrogen content was:Y(diffusion method-N)=0.978X(flow analyzer-N)+0.502(r2=0.9755,P<0.01),the total nitrogen measured by the two methods was extremely significant correlation.The regression equation of improved method and molybdenum-antimony colorimetric method to determine the total phosphorus content was:Y(molybdenum-antimony colorimetric method-P)=0.985 X(flow analyzer-P)+0.035(r2=0.9835,P<0.01),the total phosphorus measured by the two methods was extremdy significant correlation.The improved methods for determing the total nitrogen and total phosphorus content of plants with a flow analyzer had standard addtion recovery rates of 98.23%~100.68%and 98.67%~99.11%,respectively.The relative standard deviation of the total nitrogen and total phosphorus content of three samples were repeatedly measured for four times were less than 6%.The improved method of simultaneous determination of total nitrogen and total phosphorus by flow analyzer had the advantages of simple operation,high precision and accuracy,less reagent consumption and high detection efficiency,which was suitable for the detection and analysis of large quantities of plants.