文献类型：期刊文献

中文题名：巨大芽胞杆菌在3种固沙植物根部的定殖

英文题名：Bacillus megaterium Colonization in Roots on Three Sand-Fixing Plants

作者：王天龙[1,2] 李永[1] 王曦茁[1] 郭民伟[1] 张晓娟[3] 汪来发[1] 朴春根[1]

第一作者：王天龙

机构：[1]中国林业科学研究院森林生态环境与保护研究所,国家林业局森林保护学重点试验室;[2]四川农业大学林学园艺学院;[3]内蒙古鄂尔多斯林业治沙科学研究所

年份：2010

期号：21

起止页码：90-93

中文期刊名：中国农学通报

外文期刊名：Chinese Agricultural Science Bulletin

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD_E2011_2012】；

基金：国家科技基础条件平台建设项目"林业微生物资源标准化整理;整合及共享试点"(2005DKA21207)

语种：中文

中文关键词：巨大芽孢杆菌;定殖;根际效应;固沙植物

外文关键词：Bacillus megaterium; colonization; rhizosphere effect; sand-fixing plants

分类号：S718.83

摘要：用200μg/mL的卡那霉素标记巨大芽胞杆菌菌株R,并获得抗药标记菌株,明确其在固沙植物杨柴、柠条和油蒿根上的定殖能力。将标记菌株制成菌液,浸种接种至杨柴、柠条和油蒿的种子,待种苗根系生长至约12cm后,测定R菌株在3种植物不同根段的定殖密度和根际效应。结果表明,标记菌株在灭菌土壤中定殖密度比在自然土中大,根系不同部位标记菌株定殖密度表现为从上到下逐渐减小,0～3cm根段标记菌株在根际的定殖密度达到最大,9～12cm根段几乎不能分离到标记菌株。3种植物中,杨柴的根际效应最显著,标记菌株在油蒿根际的定殖密度最大。
Bacillus megaterium strain R labeled by kanamycin was obtained after being marked with concentration 200 μg/mL kanamycin and the seeds of Hedysarum mongolicum,Caragana korshinskii and Artemisia ordosica were inoculated with the strain R solution by soaking it to evaluate the colonization density of R strain and rhizosphere effect of different root parts of these plants after the seeding root tip to 12 cm.The result showed that the density of R strain in sterilized soil was more than it in natural soil.The colonization density of strain R came from the root tip down gradually in different root parts,and the colonization density reached to maximum in the 0-3 cm part.The strain R could not be isolated in the 9-12 cm part.The rhizosphere effect of H.mongolicum was most prominent in these plants,and the colonization density of strain R reached to maximum in the rhizosphere of A.ordosica.