文献类型：期刊文献

英文题名：Comparison of homologous and heterologous formats in nanocolloidal gold-based immunoassays for parathion residue determination

作者：Liu, Yi H.[1] Xie, Rong[2] Guo, Yi R.[2] Zhu, Guo N.[2] Tang, Fu B.[1]

第一作者：刘毅华

通信作者：Tang, FB[1]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Fuyang 311400, Peoples R China;[2]Zhejiang Univ, Inst Pesticide & Environm Toxicol, Hangzhou 310003, Zhejiang, Peoples R China

年份：2012

卷号：47

期号：5

起止页码：475-483

外文期刊名：JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES

收录：;EI(收录号：20121414931251);Scopus(收录号：2-s2.0-84859372250);WOS:【SCI-EXPANDED(收录号:WOS:000303579200014)】；

基金：This work was supported by the Key Projects in the National Science & Technology Pillar Program during the Eleventh Five-year Plan Period (NO2009BADB9B09).

语种：英文

外文关键词：Nanocolloidal gold; lateral-flow strip assay; parathion; reaction format

摘要：The effectiveness of homologous and heterologous formats in a nanocolloidal gold-based immunoassay for pesticide residue determination was investigated. Parathion, one of the most toxic organophosphorus pesticides, was used as the target analyte. One-step homologous and heterologous test strips based on a nanocolloidal gold-labeled monoclonal antibody were developed for the rapid detection of parathion residues. The results showed that the heterologous format was more effective than the homologous format, being more sensitive, more specific to parathion and more tolerant of matrix interferences. The best competitive hapten was found to have a moderate heterology and the opposite electronic distribution to the immunizing hapten. The detection limits for parathion using the preferred heterologous strip were 1 mu g/L in water samples and 5 mu g/kg in soil and food samples.