文献类型：期刊文献

中文题名：An improved draft genome sequence of hybrid Populus alba×Populus glandulosa

作者：Xiong Huang[1,2] Song Chen[3] Xiaopeng Peng[2] Eun-Kyung Bae[4] Xinren Dai[1] Guiming Liu[5] Guanzheng Qu[3] Jae-Heung Ko[6] Hyoshin Lee[4] Su Chen[3] Quanzi Li[1,2] Mengzhu Lu[2]

第一作者：Xiong Huang

机构：[1]State Key Laboratory of Tree Genetics and Breeding,Chinese Academy of Forestry,Beijing 100091,People’s Republic of China;[2]Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,People’s Republic of China;[3]State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040,People’s Republic of China;[4]Division of Forest Biotechnology,National Institute of Forest Science,Suwon 441847,Republic of Korea;[5]Agro-Biotechnology Research Center,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,People’s Republic of China;[6]Department of Plant&Environmental New Resources,Kyung Hee University,Yongin 446701,Republic of Korea

年份：2021

卷号：32

期号：4

起止页码：1663-1672

中文期刊名：林业研究：英文版

收录：CSTPCD;;Scopus;CSCD:【CSCD2021_2022】；

基金：supported by grants CAFYBB2017ZY001 and TGB2016001 from Fundamental Research Funds of the Chinese Academy of Forestry。

语种：英文

中文关键词：Genome assembly;Gene annotation;Hybrid poplar;Populus alba×p.glandulosa cl.84 K

分类号：S792.11

摘要：Populus alba × P.glandulosa clone 84 K,derived from South Korea,is widely cultivated in China and used as a model in the molecular research of woody plants because of hi gh gene transformation efficiency.Here,we combined63-fold coverage Illumina short reads and 126-fold coverage PacBio long reads to assemble the genome.Due to the hi gh heterozygosity level at 2.1% estimated by k-mer analysis,we exploited TrioCanu for genome assembly.The PacBio clean subreads of P.alba × P.glandulosa were separated into two parts according to the similarities,compared with the parental genomes of P.alba and P.glandulosa.The two parts of the subreads were assembled to two sets of subgenomes comprising subgenome A(405.31 Mb,from P.alba)and subgenome G(376.05 Mb,from P.glandulosa) with the contig N50 size of 5.43 Mb and 2.15 Mb,respectively.A high-quality P.alba × P.glandulosa genome assembly was obtained.The genome size was 781.36 Mb with the contig N50 size of 3.66 Mb and the longest contig was 19.47 Mb.In addition,a total of 176.95 Mb(43.7%),152.37 Mb(40.5%)of repetitive elements were identified and a total of 38,701 and 38,449 protein-coding genes were predicted in subgenomes A and G,respectively.For functional annotation,96.98% of subgenome A and 96.96% of subgenome G genes were annotated with public databases.This de novo assembled genome will facilitate systematic and comprehensive study,such as multi-omics analysis,in the model tree P.alba X P.glandulosa.