文献类型：期刊文献

英文题名：Genome-Wide Characterization of Sedum plumbizincicola HMA Gene Family Provides Functional Implications in Cadmium Response

作者：Huang, Qingyu[1,2,3] Qiu, Wenmin[2,3] Yu, Miao[2] Li, Shaocui[2] Lu, Zhuchou[2] Zhu, Yue[2] Kan, Xianzhao[1] Zhuo, Renying[2,3]

第一作者：Huang, Qingyu

通信作者：Kan, XZ[1];Zhuo, RY[2];Zhuo, RY[3]

机构：[1]Anhui Normal Univ, Coll Life Sci, Inst Bioinformat, Wuhu 241000, Peoples R China;[2]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[3]Chinese Acad Forestry, Key Lab Tree Breeding Zhejiang Prov, Res Inst Subtrop Forestry, Hangzhou 311400, Peoples R China

年份：2022

卷号：11

期号：2

外文期刊名：PLANTS-BASEL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000750559600001)】；

基金：FundingThis work was supported by the National Nonprofit Institute Research Grant of CAF (No. CAFYBB2019SZ001 RISFZ-2021-01 and CAFYBB2020SY016).

语种：英文

外文关键词：HMA family genes; Sedum plumbizincicola; evolution; nutrient deficiency; divalent metal toxicity; expression profiles; SpHMA7

摘要：Heavy-metal ATPase (HMA), an ancient family of transition metal pumps, plays important roles in the transmembrane transport of transition metals such as Cu, Zn, Cd, and Co. Although characterization of HMAs has been conducted in several plants, scarcely knowledge was revealed in Sedum plumbizincicola, a type of cadmium (Cd) hyperaccumulator found in Zhejiang, China. In this study, we first carried out research on genome-wide analysis of the HMA gene family in S. plumbizincicola and finally identified 8 SpHMA genes and divided them into two subfamilies according to sequence alignment and phylogenetic analysis. In addition, a structural analysis showed that SpHMAs were relatively conserved during evolution. All of the SpHMAs contained the HMA domain and the highly conserved motifs, such as DKTGT, GDGxNDxP, PxxK S/TGE, HP, and CPx/SPC. A promoter analysis showed that the majority of the SpHMA genes had cis-acting elements related to the abiotic stress response. The expression profiles showed that most SpHMAs exhibited tissue expression specificity and their expression can be regulated by different heavy metal stress. The members of Zn/Co/Cd/Pb subgroup (SpHMA1-3) were verified to be upregulated in various tissues when exposed to CdCl2. Here we also found that the expression of SpHMA7, which belonged to the Cu/Ag subgroup, had an upregulated trend in Cd stress. Overexpression of SpHMA7 in transgenic yeast indicated an improved sensitivity to Cd. These results provide insights into the evolutionary processes and potential functions of the HMA gene family in S. plumbizincicola, laying a theoretical basis for further studies on figuring out their roles in regulating plant responses to biotic/abiotic stresses.