文献类型：期刊文献

英文题名：Extraction of triterpene acids from loquat leaves via a novel hydrophobic deep eutectic solvent screened by COSMO-SAC model

作者：Huang, Lu[1] Guo, Yingjie[1] Jin, Tianqi[3] Yan, Ke[1] Liu, Xianglin[1] He, Shuyang[1] Li, Lin[1] Gong, Ye[3] Ma, Junyi[1] Yu, Hang[1] Lu, Zhoumin[1] Liu, Yupeng[4] Dong, Wuzi[5] Yang, Fangxia[1,2]

第一作者：Huang, Lu

通信作者：Yang, FX[1]

机构：[1]Northwest A&F Univ, Coll Forestry, Yangling 712100, Shaanxi, Peoples R China;[2]Northwest A&F Univ, Coll Forestry, Shaanxi Prov Key Lab Econ Plant Resources Dev & Ut, Yangling 712100, Shaanxi, Peoples R China;[3]Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Shaanxi, Peoples R China;[4]Chinese Acad Forestry, Inst Chem Ind Forest Prod, Natl Engn & Technol Res Ctr Forest Chem Ind, Nanjing 210037, Jiangsu, Peoples R China;[5]Shaanxi Dayi Xunlong Biotechnol Co LTD, Yangling 712100, Shaanxi, Peoples R China

年份：2023

卷号：427

外文期刊名：JOURNAL OF CLEANER PRODUCTION

收录：;EI(收录号：20234214915426);Scopus(收录号：2-s2.0-85174197080);WOS:【SCI-EXPANDED(收录号:WOS:001108246400001)】；

基金：This work was supported by the Science and Technology Program of Shaanxi Province (2022SF-512) , the Science and Technology Innovation and Achievement Transformation Program of Experimental Demonstration Station (base) of Northwest A & F University (TGZX2021-32) , and the project of Shaanxi Academy of Forestry Science (20210917000011) .

语种：英文

外文关键词：Hydrophobic deep eutectic solvent; Extraction; Triterpene acids; COSMO-SAC model; C18 reverse -phase silica gel column; chromatography

摘要：Triterpene acids (TTAs) have promising applications in functional food, medicine and other fields. Here, ultrasonic-assisted hydrophobic deep eutectic solvent (HDES) extraction was employed to gain TTAs from loquat leaves. Combining COSMO-SAC model prediction with experimental verification,the HDES, composed of thymol and benzyl alcohol (molar ratio 2:1), was proved to have the highest extraction ability for TTAs. The preliminarily optimized extraction conditions were solid to solvent ratio 1:10 (g/g), temperature 60 degree celsius and extraction time 60 min. Under these conditions, the extraction yield of ursolic acid (the main component of loquat leaf TTAs) was 17.769 +/- 0.799 mg/g dry loquat leaf. HDES and TTAs were recovered from HDES extracting solution via gradient elution (60% and 80% ethanol aqueous solution, respectively) based on C18 reverse-phase silica gel column chromatography under low pressure (0.5 MPa). Recovered HDES could be reused for extracting TTAs from loquat leaves directly and had a similar extraction performance compared to fresh HDES. The purity of the obtained crude TTAs sample was 81.18% based on the total contents of maslinic acid (MA), corosolic acid (CA), oleanolic acid (OA) and ursolic acid (UA), with a mass ratio of 11:26:10:53. Crude TTAs sample was further separated by C18 reverse-phase silica gel column chromatography using 80% ethanol aqueous solution as mobile phase under high pressure (15 Mpa). By this mean, CA sample with 69.30% purity (MA to CA mass ratio 7:18) and UA sample with 93.94% purity (OA to UA mass ratio 4:21) were obtained. The in vitro antioxidant ability order of the attained TTAs samples was CA sample > crude TTAs sample > UA sample. This study laid a good scientific basis for applying HDES and C18 reverse-phase silica gel column chromatog-raphy in the bioactive component extraction and separation fields. It also provided theoretical basis and tech-nical support for obtainment and utilization of high-purity loquat leaf TTAs in the fields of medicine, functional food, cosmetics and so on.