文献类型：期刊文献

英文题名：De novo assembly of a new Olea europaea genome accession using nanopore sequencing

作者：Rao, Guodong[1,2,3] Zhang, Jianguo[1,2,3] Liu, Xiaoxia[1] Lin, Chunfu[4] Xin, Huaigen[5] Xue, Li[1] Wang, Chenhe[1]

第一作者：Rao, Guodong;饶国栋

通信作者：Rao, GD[1];Zhang, JG[1];Rao, GD[2];Zhang, JG[2]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Nanjing Forestry Univ, Collaborat Innovat Ctr Sustainable Forestry South, Nanjing 210037, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forestry, Natl Forestry & Grassland Adm, Key Lab Tree Breeding & Cultivat, Beijing 100091, Peoples R China;[4]MIANNING Yuansheng Agr Sci & Technol Co Ltd, Chengdu 615600, Sichuan, Peoples R China;[5]Biomarker Technol Corp, Beijing 101300, Peoples R China

年份：2021

卷号：8

期号：1

外文期刊名：HORTICULTURE RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000636340500001)】；

基金：This work was supported by The National Key R&D Program of China (2018YFD1000603-3) and Fundamental Research Funds for the Central Non-profit Research Institution of Chinese Academy of Forestry (CAFYBB2018QB001). We thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript.

语种：英文

摘要：Olive (Olea europaea L.) is internationally renowned for its high-end product, extra virgin olive oil. An incomplete genome of O. europaea was previously obtained using shotgun sequencing in 2016. To further explore the genetic and breeding utilization of olive, an updated draft genome of olive was obtained using Oxford Nanopore third-generation sequencing and Hi-C technology. Seven different assembly strategies were used to assemble the final genome of 1.30Gb, with contig and scaffold N50 sizes of 4.67Mb and 42.60Mb, respectively. This greatly increased the quality of the olive genome. We assembled 1.1Gb of sequences of the total olive genome to 23 pseudochromosomes by Hi-C, and 53,518 protein-coding genes were predicted in the current assembly. Comparative genomics analyses, including gene family expansion and contraction, whole-genome replication, phylogenetic analysis, and positive selection, were performed. Based on the obtained high-quality olive genome, a total of nine gene families with 202 genes were identified in the oleuropein biosynthesis pathway, which is twice the number of genes identified from the previous data. This new accession of the olive genome is of sufficient quality for genome-wide studies on gene function in olive and has provided a foundation for the molecular breeding of olive species.